Recombinant Full Length Human RAB8A Protein, C-Flag-tagged

• Cat.No.: RAB8A-285HFL
• Product Overview: Recombinant Full Length Human RAB8A Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 23.5 kDa
• AA Sequence: MAKTYDYLFKLLLIGDSGVGKTCVL FRFSEDAFNSTFISTIGIDFKIRTI ELDGKRIKLQIWDTAGQERF RTITTAYYRGAMGIMLVYDITNEKS FDNIRNWIRNIEEHASADVEKMILG NKCDVNDKRQVSKERGEKLA LDYGIKFMETSAKANINVENAFFTL ARDIKAKMDKKLEGNSPQGSNQGVK ITPDQQKRSSFFRCVLLTRTRPLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transcription Factors

Gene Information

• Gene Name: RAB8A RAB8A, member RAS oncogene family [ Homo sapiens (human) ]
• Official Symbol: RAB8A
• Synonyms: MEL; RAB8
• Gene ID: 4218
• mRNA Refseq: NM_005370.5
• Protein Refseq: NP_005361.2
• MIM: 165040
• UniProt ID: P61006