Recombinant Full Length Human TLE1 Protein, C-Flag-tagged

• Cat.No.: TLE1-860HFL
• Product Overview: Recombinant Full Length Human TLE1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Enables identical protein binding activity and transcription corepressor activity. Involved in negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of anoikis; and regulation of gene expression. Located in cytosol and nucleoplasm. Part of beta-catenin-TCF complex.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 83 kDa
• AA Sequence: MFPQSRHPTPHQAAGQPFKFTIPES LDRIKEEFQFLQAQYHSLKLECEKL ASEKTEMQRHYVMYYEMSYG LNIEMHKQTEIAKRLNTICAQVIPF LSQEHQQQVAQAVERAKQVTMAELN AIIGQQQLQAQHLSHGHGPP VPLTPHPSGLQPPGIPPLGGSAGLL ALSSALSGQSHLAIKDDKKHHDAEH HRDREPGTSNSLLVPDSLRG TDKRRNGPEFSNDIKKRKVDDKDSS HYDSDGDKSDDNLVVDVSNEDPSSP RASPAHSPRENGIDKNRLLK KDASSSPASTASSASSTSLKSKEMS LHEKASTPVLKSSTPTPRSDMPTPG TSATPGLRPGLGKPPAIDPL VNQAAAGLRTPLAVPGPYPAPFGMV PHAGMNGELTSPGAAYASLHNMSPQ MSAAAAAAAVVAYGRSPMVG FDPPPHMRVPTIPPNLAGIPGGKPA YSFHVTADGQMQPVPFPPDALIGPG IPRHARQINTLNHGEVVCAV TISNPTRHVYTGGKGCVKVWDISHP GNKSPVSQLDCLNRDNYIRSCKLLP DGCTLIVGGEASTLSIWDLA APTPRIKAELTSSAPACYALAISPD SKVCFSCCSDGNIAVWDLHNQTLVR QFQGHTDGASCIDISNDGTK LWTGGLDNTVRSWDLREGRQLQQHD FTSQIFSLGYCPTGEWLAVGMESSN VEVLHVNKPDKYQLHLHESC VLSLKFAYCGKWFVSTGKDNLLNAW RTPYGASIFQSKESSSVLSCDISVD DKYIVTGSGDKKATVYEVIYTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transcription Factors

Gene Information

• Gene Name: TLE1 TLE family member 1, transcriptional corepressor [ Homo sapiens (human) ]
• Official Symbol: TLE1
• Synonyms: ESG; ESG1; GRG1; TLE-1
• Gene ID: 7088
• mRNA Refseq: NM_005077.5
• Protein Refseq: NP_005068.2
• MIM: 600189
• UniProt ID: Q04724