Recombinant Full Length Mouse Atp-Binding Cassette Sub-Family G Member 1(Abcg1) Protein, His-Tagged

• Cat.No.: RFL31234MF
• Product Overview: Recombinant Full Length Mouse ATP-binding cassette sub-family G member 1(Abcg1) Protein (Q64343) (1-666aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Mus musculus (Mouse)
• Tag: His
• Form: Lyophilized powder
• Protein Length: Full Length (1-666)
• AA Sequence: MACLMAAFSVGTAMNASSYSAAMTE PKSVCVSVDEVVSSNVDEVETDLLN GHLKKVDNNF TEAQRFSSLPRRAAVNIEFKDLSYS VPEGPWWKKKGYKTLLKGISGKFNS GELVAIMGPS GAGKSTLMNILAGYRETGMKGAVLI NGMPRDLRCFRKVSCYIMQDDMLLP HLTVQEAMMV SAHLKLQEKDEGRREMVKEILTALG LLPCANTRTGSLSGGQRKRLAIALE LVNNPPVMFF DEPTSGLDSASCFQVVSLMKGLAQG GRSIVCTIHQPSAKLFELFDQLYVL SQGQCVYRGK VSNLVPYLRDLGLNCPTYHNPADFV MEVASGEYGDQNSRLVRAVREGMCD ADYKRDLGGD TDVNPFLWHRPAEEDSASMEGCHSF SASCLTQFCILFKRTFLSIMRDSVL THLRITSHIG IGLLIGLLYLGIGNEAKKVLSNSGF LFFSMLFLMFAALMPTVLTFPLEMS VFLREHLNYW YSLKAYYLAKTMADVPFQIMFPVAY CSIVYWMTSQPSDAVRFVLFAALGT MTSLVAQSLG LLIGAASTSLQVATFVGPVTAIPVL LFSGFFVSFDTIPAYLQWMSYISYV RYGFEGVILS IYGLDREDLHCDIAETCHFQKSEAI LRELDVENAKLYLDFIVLGIFFISL RLIAYFVLRY KIRAER
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: Abcg1
• Synonyms: Abcg1; Abc8; Wht1; ATP-binding cassette sub-family G member 1; ATP-binding cassette transporter 8; White protein homolog
• UniProt ID: Q64343

Gene Information

• Gene Name: Abcg1
• Synonyms: Abcg1; Abc8; Wht1; ATP-binding cassette sub-family G member 1; ATP-binding cassette transporter 8; White protein homolog
• UniProt ID: Q64343

Reviews

11/03/2017

I have been consistently impressed by the reliability and consistency of the protein product

10/31/2017

It effectively interacting with other molecules as expected.

10/02/2017

The protein product was easy to work with.

11/04/2016

The protein product displayed excellent stability.

05/11/2016

Their experts offering valuable insights and troubleshooting advice for my experiments.

Q&As

How does ABCG1 contribute to the prevention of atherosclerosis and the development of cardiovascular diseases?

ABCG1 contributes to the prevention of atherosclerosis.ABCG1 helps reduce the formation of foam cells, which are a key component of atherosclerotic plaques. By reducing foam cell formation and lipid deposition, ABCG1 can help protect against the development and progression of atherosclerosis.

How is ABCG1 expression regulated at the transcriptional level in response to changes in cellular cholesterol levels?

ABCG1 expression is regulated at the transcriptional level in response to changes in cellular cholesterol levels. Sterol regulatory element-binding proteins (SREBPs) are key transcription factors involved in this regulation. When cellular cholesterol levels are low, SREBPs are activated, leading to the upregulation of ABCG1 expression. 

What are the mechanisms by which ABCG1 contributes to lipid metabolism and influences lipid-related disorders beyond atherosclerosis?

ABCG1 has been linked to cellular processes like cell proliferation, apoptosis, and autophagy, indicating its involvement in broader cellular functions.

Does ABCG1 interact with other lipid transporters or membrane proteins to facilitate lipid efflux and cellular homeostasis?

ABCG1 is known to play a role in immune cells, particularly macrophages. It contributes to cholesterol homeostasis in macrophages, which is crucial for their proper function in the immune response.

Are there any specific signaling pathways or protein-protein interactions that regulate ABCG1 activity or intracellular trafficking?

 Several signaling pathways and protein-protein interactions are involved in the regulation of ABCG1 activity and intracellular trafficking. The activation of nuclear receptors, such as liver X receptors (LXRs) and peroxisome proliferator-activated receptors (PPARs), can upregulate ABCG1 expression. 

What is the role of ABCG1 in cellular lipid metabolism and cholesterol homeostasis?

ABCG1 functions as a transporter protein that facilitates the efflux of cellular lipids, particularly cholesterol and phospholipids, to maintain lipid balance. By promoting the efflux of excess cholesterol, ABCG1 helps prevent cholesterol accumulation and the formation of lipid droplets within cells.

What diseases will be developed with dysregulation of ABCG1?

Dysregulation of ABCG1 in immune cells can affect inflammatory processes and the development of diseases such as atherosclerosis.

Are there any known genetic variations or polymorphisms in the ABCG1 gene that are associated with altered lipid profiles or increased cardiovascular disease risk?

Yes, there are known genetic variations and polymorphisms in the ABCG1 gene that have been associated with altered lipid profiles and an increased risk of cardiovascular disease. 

What are the specific lipid substrates transported by ABCG1, and what are the extracellular acceptors involved in lipid efflux?

The specific lipid substrates transported by ABCG1 are primarily cholesterol and phospholipids. ABCG1 promotes the efflux of these lipids from cells to extracellular acceptors, such as high-density lipoprotein (HDL) particles

What type of protein is ABCG1?

ABCG1 (ATP-binding cassette subfamily G member 1) is a member of the ATP-binding cassette (ABC) transporter superfamily.

How does ABCG1 expression differ among different cell types and tissues?

The expression of ABCG1 can vary among different cell types and tissues. For example,ABCG1 is highly expressed in macrophages while low in liver.

What is the fucntion of ABCG1 protein?

It plays a crucial role in cellular lipid homeostasis by mediating the efflux of lipids, primarily cholesterol and phospholipids, from cells. ABCG1 is involved in maintaining cholesterol balance and regulating lipid metabolism.

Does ABCG1 have any non-lipid-related functions or interactions with cellular processes beyond lipid transport?

These include synthetic agonists for nuclear receptors, such as liver X receptors (LXRs) and peroxisome proliferator-activated receptors (PPARs), which can enhance ABCG1 expression. 

What is the subcellular localization of ABCG1 and are there any mechanisms that regulate its localization?

ABCG1 is predominantly found in the plasma membrane but can also be localized to intracellular compartments, such as endosomes and the Golgi apparatus. Its subcellular distribution may vary depending on the cell type and physiological conditions.

Does ABCG1 play a role in foam cell formation and the pathogenesis of atherosclerotic plaques?

Yes, ABCG1 plays a role in foam cell formation and the pathogenesis of atherosclerotic plaques. Foam cells are formed when macrophages in arterial walls take up excessive cholesterol and become overloaded with lipids. 

What is the role of ABCG1 in immune cells and its impact on inflammatory processes?

Phosphorylation of specific serine residues has been shown to modulate ABCG1 function and its interaction with other proteins

What fcators regulate ABCG1 expression?

ABCG1 expression is regulated by various factors, including sterol regulatory element-binding proteins (SREBPs), liver X receptors (LXRs), and peroxisome proliferator-activated receptors (PPARs

How does function of ABCG1 differ among different cell types and tissues?

The distribution and activity of ABCG1 can be influenced by cell-specific regulatory factors and physiological conditions.

Are there any pharmacological agents or compounds that modulate ABCG1 expression or activity?

ABCG1 can interact with other lipid transporters, membrane proteins, and signaling molecules to facilitate lipid efflux and maintain cellular homeostasis. 

What is the full name of the ABCG1 protein?

The full name of ABCG1 is ATP-binding cassette subfamily G member 1.