Recombinant Full Length Mouse Type-1A Angiotensin Ii Receptor(Agtr1A) Protein, His-Tagged

• Cat.No.: RFL-2154MF
• Product Overview: Recombinant Full Length Mouse Type-1A angiotensin II receptor(Agtr1a) Protein (P29754) (1-359aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Mus musculus (Mouse)
• Tag: His
• Form: Lyophilized powder
• Protein length: Full Length (1-359AA)
• AA Sequence: MALNSSTEDGIKRIQDDCPRAGRHS YIFVMIPTLYSIIFVVGIFGNSLVV IVIYFYMKLK TVASVFLLNLALADLCFLLTLPLWA VYTAMEYRWPFGNHLCKIASASVSF NLYASVFLLT CLSIDRYLAIVHPMKSRLRRTMLVA KVTCIIIWLMAGLASLPAVIHRNVY FIENTNITVC AFHYESRNSTLPIGLGLTKNILGFL FPFLIILTSYTLIWKALKKAYEIQK NKPRNDDIFR IIMAIVLFFFFSWVPHQIFTFLDVL IQLGVIHDCKIADIVDTAMPITICI AYFNNCLNPL FYGFLGKKFKKYFLQLLKYIPPKAK SHSSLSTKMSTLSYRPSDNMSSAAK KPASCSEVE
• Purity: Greater than 90% as determined by SDS-PAGE.
• Applications: SDS-PAGE
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: Agtr1a
• Synonyms: Agtr1a; Agtr1Type-1A angiotensin II receptor; Angiotensin II type-1A receptor; AT1A
• UniProt ID: P29754

Gene Information

• Gene Name: Agtr1a
• Synonyms: Agtr1a; Agtr1Type-1A angiotensin II receptor; Angiotensin II type-1A receptor; AT1A
• UniProt ID: P29754

Reviews

07/22/2022

The customer service was fantastic—helpful, friendly, and made the ordering process a breeze!

09/18/2021

Quick delivery and secure packaging ensured my recombinant proteins arrived in perfect condition.

04/10/2021

Super impressed with the reliable recombinant proteins I received at such a reasonable cost

02/24/2021

The stability of this protein is unparalleled. It remains intact and active even under challenging conditions, ensuring reliable and reproducible results.

08/20/2020

Couldn't be happier with the high-quality recombinant proteins I got at such an amazing price.

Q&As

How does AGTR1A influence vascular tone?

AGTR1A activation leads to vasoconstriction by stimulating smooth muscle cell contraction, thereby affecting vascular tone and blood pressure.

How can protein-protein interactions involving AGTR1A be studied in the lab?

Techniques such as co-immunoprecipitation (Co-IP) or proximity-based assays like the fluorescence resonance energy transfer (FRET) can be utilized to investigate protein-protein interactions involving AGTR1A.

Are there specific ligands or agonists for AGTR1A?

Angiotensin II is the primary ligand for AGTR1A, but there are also selective agonists and antagonists that can modulate its activity for research and therapeutic purposes.

Can AGTR1A polymorphisms influence drug response?

Genetic polymorphisms in AGTR1A have been associated with variable responses to ARBs and other antihypertensive medications, affecting treatment outcomes.

How can AGTR1A signaling pathways be investigated in the lab?

Lab researchers can use techniques like phospho-specific antibodies, ELISA assays, or luciferase reporter assays to assess the activation and downstream signaling of AGTR1A pathways.

Can AGTR1A contribute to insulin resistance and metabolic disorders?

AGTR1A signaling has been implicated in insulin resistance, glucose metabolism, and the development of metabolic disorders such as obesity and diabetes.

What signaling pathways are activated by AGTR1A?

AGTR1A activates various signaling pathways, including phospholipase C (PLC), protein kinase C (PKC), mitogen-activated protein kinases (MAPKs), and intracellular calcium release.

What are the structural features of AGTR1A that allow ligand binding?

AGTR1A has extracellular domains and specific amino acid residues within the transmembrane region that facilitate angiotensin II binding and receptor activation.

How is AGTR1A activated?

AGTR1A is activated when angiotensin II binds to the receptor, leading to conformational changes that initiate intracellular signaling cascades.

What is the process for screening compounds targeting AGTR1A in the lab?

High-throughput screening (HTS) methods, such as cell-based assays or binding assays using radiolabeled ligands, can be employed to identify and evaluate potential compounds targeting AGTR1A.