Recombinant Full Length Rat Atp-Binding Cassette Sub-Family B Member 8, Mitochondrial(Abcb8) Protein, His-Tagged

• Cat.No.: RFL13678RF
• Product Overview: Recombinant Full Length Rat ATP-binding cassette sub-family B member 8, mitochondrial(Abcb8) Protein (Q5RKI8) (39-714aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Rattus norvegicus (Rat)
• Tag: His
• Form: Lyophilized powder
• Protein Length: Full Length of Mature Protein (39-714)
• AA Sequence: SSCLLRAVAQLRSQLRAHLPRSPPA SHRSTSAWCWVGGTLVVPAVLWQHP RFCLIALCEA KGSPPAQPTRARELRFKWKLFWHFL HPHLLALGLAIVLALGAALVNVQIP LLLGQLVEIV AKYTREHVGSFVSESRRLSIQLLLL YGVQGLLTFGYLVLLSHMGERMAMD MRKALFSSLL RQDIAFFDAKKTGQLVSRLTTDVQE FKSSFKLVISQGLRSSTQVIGSLMT LSILSPRLTL MLAVVTPALMGVGTLMGSGLRKLSR QCQEQIARATGVADEALGSVRTVRA FAMEKREEER YQAELESCCCKAEELGRGIALFQGL SNIAFNCMVLGTLFIGGSLVAGQQL KGGDLMSFLV ASQTVQRSMASLSVLFGQVVRGLSA GARVFEYMSLSPVIPLTGGYSIPSK DLRGSITFQN VSFSYPCRPGFNVLKNFTLKLPPGK IVALVGQSGGGKTTVASLLERFYDP TAGVVTLDGH DLRTLDPSWLRGQVIGFISQEPVLF ATTIMENIRFGKLDASDEEVYTAAR KANAHEFISS FPDGYSTVVGERGTTLSGGQKQRLA IARALIKRPTVLILDEATSALDAES ERIVQEALDR ASAGRTVLVIAHRLSTVRAAHSIIV MANGQVCEAGTHEELLQKGGLYAEL IRRQALDASL PSAPPAEKPEDHRSCQ
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: Abcb8
• Synonyms: Abcb8; Mitosur; Mitochondrial potassium channel ATP-binding subunit; ATP-binding cassette sub-family B member 8, mitochondrial; ABCB8; Mitochondrial sulfonylurea-receptor; MITOSUR
• UniProt ID: Q5RKI8

Gene Information

• Gene Name: Abcb8
• Synonyms: Abcb8; Mitosur; Mitochondrial potassium channel ATP-binding subunit; ATP-binding cassette sub-family B member 8, mitochondrial; ABCB8; Mitochondrial sulfonylurea-receptor; MITOSUR
• UniProt ID: Q5RKI8

Reviews

04/22/2022

The technicians are very professional and provided me with some useful suggestions related to experiments.

09/06/2021

The customized communication was very smooth, and I also received my customized protein products very quickly.

Q&As

Do you offer ABCB8 proteins with other types of tags?

We provide tag customization services, and you can communicate with us about specific customization needs at any time.

Can ABCB8-2327Z be customized with higher purity protein?

Yes, we can customize proteins with different purities according to your needs.

Does adding tags affect the activity of ABCB8 protein?

In general, adding small tags has very little effect on protein activity, but we cannot confirm the specific effect of adding tags on protein activity.

How much is the price of custom-made ABCB8?

The specific price will vary depending on the customization needs. You can communicate with us about the specific customization needs first, and then we will provide you with price information

ABCB8-2464H has two types of freeze-dried powder and liquid, can you send me the liquid?

Yes, you put forward this demand when purchasing, and we will deliver according to your demand.

Can the endotoxin level of ABCB8 be customized?

Yes, we can customize protein products with different endotoxin levels.

Does ABCB8-1095M participate in this promotion?

Yes, this product is eligible for this promotion.

How is protein purification achieved?

Our commonly used purification methods include hydrophobic chromatography, affinity chromatography, ion exchange chromatography, salting out, molecular sieve, etc.

I want to buy ABCB8 protein for WB. Can you provide the WB protocol?

You can make this request when purchasing, and we will send you the relevant protocol.

What is the effect of adding trehalose in storage buffer?

Trehalose can prevent protein secondary structure changes, elongation and aggregation during freeze-drying.

How long does it take for the product to arrive after purchase?

After the customer purchases the product, we will ship it as soon as possible, but the arrival time is related to your specific location. If it is a custom-made product, the delivery time will be longer.

I use ABCB8 protein for western blotting, use skim milk to block and incubate the primary antibody at 4°C, how long is the normal incubation time?

Incubate with the primary antibody overnight at 4°C.