Recombinant Human ABCB8 293 Cell Lysate

• Cat.No.: ABCB8-9151HCL

Specification

• Description: Antigen standard for ATP-binding cassette, sub-family B (MDR/TAP), member 8 (ABCB8), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ABCB8 ATP-binding cassette, sub-family B (MDR/TAP), member 8 [ Homo sapiens ]
• Official Symbol: ABCB8
• Synonyms: ABCB8; ATP-binding cassette, sub-family B (MDR/TAP), member 8; ATP-binding cassette sub-family B member 8, mitochondrial; EST328128; M ABC1; MABC1; mitochondrial ABC protein; mitochondrial ATP-binding cassette 1; M-ABC1
• Gene ID: 11194
• mRNA Refseq: NM_007188
• Protein Refseq: NP_009119
• MIM: 605464
• UniProt ID: Q9NUT2
• Chromosome Location: 7q35-q36
• Pathway: ABC transporters, organism-specific biosystem; ABC transporters, conserved biosystem; ABC-family proteins mediated transport, organism-specific biosystem; Mitochondrial ABC transporters, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem
• Function: ATP binding; ATPase activity; ATPase activity, coupled to transmembrane movement of substances; nucleotide binding; transporter activity

Reviews

04/22/2022

The technicians are very professional and provided me with some useful suggestions related to experiments.

09/06/2021

The customized communication was very smooth, and I also received my customized protein products very quickly.

Q&As

Do you offer ABCB8 proteins with other types of tags?

We provide tag customization services, and you can communicate with us about specific customization needs at any time.

Can ABCB8-2327Z be customized with higher purity protein?

Yes, we can customize proteins with different purities according to your needs.

Does adding tags affect the activity of ABCB8 protein?

In general, adding small tags has very little effect on protein activity, but we cannot confirm the specific effect of adding tags on protein activity.

How much is the price of custom-made ABCB8?

The specific price will vary depending on the customization needs. You can communicate with us about the specific customization needs first, and then we will provide you with price information

ABCB8-2464H has two types of freeze-dried powder and liquid, can you send me the liquid?

Yes, you put forward this demand when purchasing, and we will deliver according to your demand.

Can the endotoxin level of ABCB8 be customized?

Yes, we can customize protein products with different endotoxin levels.

Does ABCB8-1095M participate in this promotion?

Yes, this product is eligible for this promotion.

How is protein purification achieved?

Our commonly used purification methods include hydrophobic chromatography, affinity chromatography, ion exchange chromatography, salting out, molecular sieve, etc.

I want to buy ABCB8 protein for WB. Can you provide the WB protocol?

You can make this request when purchasing, and we will send you the relevant protocol.

What is the effect of adding trehalose in storage buffer?

Trehalose can prevent protein secondary structure changes, elongation and aggregation during freeze-drying.

How long does it take for the product to arrive after purchase?

After the customer purchases the product, we will ship it as soon as possible, but the arrival time is related to your specific location. If it is a custom-made product, the delivery time will be longer.

I use ABCB8 protein for western blotting, use skim milk to block and incubate the primary antibody at 4°C, how long is the normal incubation time?

Incubate with the primary antibody overnight at 4°C.