Recombinant Full Length Variola Virus Hemagglutinin(Ha) Protein, His-Tagged

• Cat.No.: RFL520VF
• Product Overview: Recombinant Full Length Variola virus Hemagglutinin(HA) Protein (P33807) (17-313aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
• Tag: His
• Form: Lyophilized powder
• Protein length: Full Length of Mature Protein (17-313)
• AA Sequence: TPYPQTQISKKIGDDATLSCSRNNI NDYVVMSAWYKEPNSIILLAAKSDV LYFDNYTKDK ISYDSPYDDLVTTITIKSLTAKDAG TYVCAFFMTSTTNDTDKVDYEEYST ELIVNTDSES TIDIILSGSSHSPETSSEKPDYINN FNCSLVFEIATPGPITDNVENHTDT VTYTSDIINT VSTSSRESTTVKTSGPITNKEDHTV TDTVSYTTVSTSSEIVTTKSTANDA HNDNEPSTVS PTTVKNITKSIGKYSTKDYVKVFGI AALIILSAVAIFCITYYICNKRSRK YKTENKV
• Purity: Greater than 90% as determined by SDS-PAGE.
• Applications: SDS-PAGE
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: HA
• Synonyms: HA; A56R; J9R; Protein A56; Hemagglutinin
• UniProt ID: P33807

Gene Information

• Gene Name: HA
• Synonyms: HA; A56R; J9R; Protein A56; Hemagglutinin
• UniProt ID: P33807

Reviews

06/01/2021

The transport process does not affect the protein quality, and the stability is good.

11/27/2019

Higher solubility makes it easy to prepare the desired concentration.

Q&As

How about the structural characteristics of HA protein?

HA protein is a glycoprotein that contains thousands of carbohydrates and an amino acid chain consisting of 566 amino acid residues. The structure of HA protein has triple symmetry, consisting of three monomers that form a spiral.

Will genetic variants in HA proteins affect vaccine development?

Genetic variants in the HA protein may affect the development and production of influenza vaccines. Because HA protein is an important component of influenza viruses, different subtypes or variants of HA protein may require different vaccines to prepare.

Why is hemagglutinin protein related to viral replication?

This protein is one of the key factors in the replication process of influenza viruses. When a virus infects a host cell, the receptor-binding region of the HA protein binds to the receptor on the cell surface, allowing the virus to invade the host cell and replicate.

What are the subtypes of HA protein?

There are currently 18 known HA subtypes of influenza viruses, H1-H18. Among them, the H1, H2, H3, H5, H7, and H9 subtypes are associated with human viruses, while other subtypes are generally associated with animal viruses.

What are the physiological functions of HA protein?

The main physiological function of HA protein is to bind to host cells and mediate the entry of influenza viruses into host cells. It contains specific regions that bind glycoprotein receptors that allow the influenza virus to adhere to them. This is an important link that allows the virus to stably bind and enter the cell.

What laboratory techniques are HA proteins used in?

HA protein is widely used in viral research and drug development. Researchers can use HA proteins to test drugs such as influenza virus activators, antibodies and vaccines, and HA proteins are also widely used to develop novel immunoassays and treatments.