Recombinant Human ABCB8 protein, His & T7-tagged

• Cat.No.: ABCB8-8128H
• Product Overview: Recombinant Human ABCB8 aa. (Val472~Ser735 (Accession # Q9NUT2)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 32.4kDa.
• Protein length: Val472~Ser735 (Accession # Q9NUT2)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.2.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: ABCB8 ATP binding cassette subfamily B member 8 [ Homo sapiens (human) ]
• Official Symbol: ABCB8
• Synonyms: ABCB8; ATP binding cassette subfamily B member 8; MABC1; M-ABC1; EST328128; ATP-binding cassette sub-family B member 8, mitochondrial; ATP-binding cassette, sub-family B (MDR/TAP), member 8; mitochondrial ABC protein; mitochondrial ATP-binding cassette 1
• Gene ID: 11194
• mRNA Refseq: NM_001282291.1
• Protein Refseq: NP_001269220.1
• UniProt ID: Q9NUT2

Reviews

04/22/2022

The technicians are very professional and provided me with some useful suggestions related to experiments.

09/06/2021

The customized communication was very smooth, and I also received my customized protein products very quickly.

Q&As

Do you offer ABCB8 proteins with other types of tags?

We provide tag customization services, and you can communicate with us about specific customization needs at any time.

Can ABCB8-2327Z be customized with higher purity protein?

Yes, we can customize proteins with different purities according to your needs.

Does adding tags affect the activity of ABCB8 protein?

In general, adding small tags has very little effect on protein activity, but we cannot confirm the specific effect of adding tags on protein activity.

How much is the price of custom-made ABCB8?

The specific price will vary depending on the customization needs. You can communicate with us about the specific customization needs first, and then we will provide you with price information

ABCB8-2464H has two types of freeze-dried powder and liquid, can you send me the liquid?

Yes, you put forward this demand when purchasing, and we will deliver according to your demand.

Can the endotoxin level of ABCB8 be customized?

Yes, we can customize protein products with different endotoxin levels.

Does ABCB8-1095M participate in this promotion?

Yes, this product is eligible for this promotion.

How is protein purification achieved?

Our commonly used purification methods include hydrophobic chromatography, affinity chromatography, ion exchange chromatography, salting out, molecular sieve, etc.

I want to buy ABCB8 protein for WB. Can you provide the WB protocol?

You can make this request when purchasing, and we will send you the relevant protocol.

What is the effect of adding trehalose in storage buffer?

Trehalose can prevent protein secondary structure changes, elongation and aggregation during freeze-drying.

How long does it take for the product to arrive after purchase?

After the customer purchases the product, we will ship it as soon as possible, but the arrival time is related to your specific location. If it is a custom-made product, the delivery time will be longer.

I use ABCB8 protein for western blotting, use skim milk to block and incubate the primary antibody at 4°C, how long is the normal incubation time?

Incubate with the primary antibody overnight at 4°C.