Recombinant Human ABHD6 293 Cell Lysate

• Cat.No.: ABHD6-9131HCL

Specification

• Description: Antigen standard for abhydrolase domain containing 6 (ABHD6) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ABHD6 abhydrolase domain containing 6 [ Homo sapiens ]
• Official Symbol: ABHD6
• Gene ID: 57406
• mRNA Refseq: NM_020676.5
• Protein Refseq: NP_065727.4
• UniProt ID: Q9BV23
• Chromosome Location: 3p14.3
• Pathway: Retrograde endocannabinoid signaling, organism-specific biosystem;Retrograde endocannabinoid signaling, conserved biosystem;triacylglycerol degradation, organism-specific biosystem
• Function: acylglycerol lipase activity

Reviews

10/13/2020

Your ABHD6 recombinant protein helped me make some progress in epilepsy research.

Q&As

I want to buy ABHD6 protein with catalytic triad mutation, any suggestions?

Our recombinant ABHD6 protein contains the catalytic triad responsible for 2-AG hydrolysis and can be mutated for amino acids to suit your specific demands.

Which protein family does ABHD6 belong to?

ABHD6 is an integral membrane protein that belongs to the serine hydrolase family.

What diseases is ABHD6 associated with?

As a member of the endocannabinoid signaling system, ABHD6 is associated with several devastating diseases including epilepsy.

Where is ABHD6 most expressed?

Highly expressed in the immune system, especially the spleen and small intestine, then the brain (particularly the cerebral cortex, pituitary gland and hippocampus), in addition, ABHD6 expression in selected cell types may vary with development and sex

There is no ABHD6 protein I want in the product catalog, can it be customized?

Of course, we support custom protein production services.

What tag does your ABHD6 recombinant protein use?

To facilitate affinity chromatography, our ABHD6 proteins are available with your choice of GST, His, or Myc/DDK tags. We can also use other tags depending on your research needs.

Which expression systems do you use for your recombinant ABHD6 protein?

We provide a variety of expression systems including E.coli, HEK293, wheat germ, in vitro cell free system and mammalian cells.