Recombinant Human ACAA2 293 Cell Lysate

• Cat.No.: ACAA2-9118HCL

Specification

• Description: Antigen standard for acetyl-Coenzyme A acyltransferase 2 (ACAA2), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ACAA2 acetyl-CoA acyltransferase 2 [ Homo sapiens ]
• Official Symbol: ACAA2
• Synonyms: ACAA2; acetyl-CoA acyltransferase 2; acetyl Coenzyme A acyltransferase 2; 3-ketoacyl-CoA thiolase, mitochondrial; DSAEC; mitochondrial 3 oxoacyl Coenzyme A thiolase; T1; beta ketothiolase; beta-ketothiolase; acetyl-Coenzyme A acyltransferase 2; mitochondrial 3-oxoacyl-CoA thiolase; mitochondrial 3-oxoacyl-Coenzyme A thiolase; FLJ35992; FLJ95265
• Gene ID: 10449
• mRNA Refseq: NM_006111
• Protein Refseq: NP_006102
• MIM: 604770
• UniProt ID: P42765
• Chromosome Location: 18q21
• Pathway: Fatty Acid Biosynthesis, organism-specific biosystem; Fatty acid biosynthesis, elongation, mitochondria, organism-specific biosystem; Fatty acid biosynthesis, elongation, mitochondria, conserved biosystem; Fatty acid elongation, organism-specific biosystem; Fatty acid elongation, conserved biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem
• Function: acetyl-CoA C-acyltransferase activity; protein binding; transferase activity, transferring acyl groups other than amino-acyl groups

Reviews

02/22/2021

Highly effective in detecting and measuring the activity of protein kinases in kinase assays.

01/21/2021

Provides consistent and accurate quantification of the target protein in different assay formats.

04/05/2017

Shows excellent performance in detecting and measuring the activity of proteases in protease assays.

Q&As

What is the molecular weight of ACAA2 protein?

The molecular weight of ACAA2 protein is approximately 45 kDa.

How does ACAA2 contribute to the pathogenesis of certain types of cancer?

ACAA2 expression is upregulated in some types of cancer, including prostate and ovarian cancer.

How is ACAA2 protein regulated at the transcriptional level?

ACAA2 protein is regulated at the transcriptional level by multiple factors, including PPARs and SREBP-1c.

Where is ACAA2 predominantly expressed in the human body?

ACAA2 is predominantly expressed in the liver and adipose tissue.

What is the clinical significance of ACAA2 protein in human diseases and disorders?

ACAA2 may have clinical significance in the diagnosis and treatment of metabolic disorders and certain types of cancer.

What is the primary function of acetyl-CoA acyltransferase 2 (ACAA2) protein?

ACAA2 protein's primary function is to participate in fatty acid and ketone body metabolism.

What is the role of ACAA2 in the development of metabolic diseases, such as obesity and diabetes?

ACAA2 has been implicated in the development of metabolic disorders, including obesity and type 2 diabetes.

What are the known inhibitors or activators of ACAA2 protein?

Some known inhibitors of ACAA2 include hexachlorophene and fusicoccin-A, while known activators include medium-chain fatty acids and certain drugs.

How does ACAA2 participate in the metabolism of fatty acids and ketone bodies?

ACAA2 catalyzes the transfer of acyl groups between CoA and acyl-CoA substrates.

What is the three-dimensional structure of ACAA2 protein?

The three-dimensional structure of ACAA2 protein is characterized by an alpha/beta hydrolase fold.