Recombinant Human ACADM 293 Cell Lysate

• Cat.No.: ACADM-9114HCL

Specification

• Description: Antigen standard for acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain (ACADM), nuclear gene encoding mitochondrial protein, transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ACADM acyl-CoA dehydrogenase, C-4 to C-12 straight chain [ Homo sapiens ]
• Official Symbol: ACADM
• Synonyms: ACADM; acyl-CoA dehydrogenase, C-4 to C-12 straight chain; acyl Coenzyme A dehydrogenase, C 4 to C 12 straight chain; medium-chain specific acyl-CoA dehydrogenase, mitochondrial; ACAD1; MCAD; MCADH; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
• Gene ID: 34
• mRNA Refseq: NM_000016
• Protein Refseq: NP_000007
• MIM: 607008
• UniProt ID: P11310
• Chromosome Location: 1p31
• Pathway: Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA, organism-specific biosystem; Beta oxidation of octanoyl-CoA to hexanoyl-CoA, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Fatty Acid Beta Oxidation, organism-specific biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem
• Function: acryloyl-CoA reductase activity; acyl-CoA dehydrogenase activity; acyl-CoA dehydrogenase activity; acyl-CoA dehydrogenase activity; acyl-CoA dehydrogenase activity; flavin adenine dinucleotide binding; identical protein binding; medium-chain-acyl-CoA dehydrogenase activity

Reviews

04/29/2022

Demonstrates excellent stability and consistency in long-term storage.

05/25/2018

Highly effective in detecting post-translational modifications of the target protein.

Q&As

How does the activity of this protein contribute to cellular metabolism?

The activity of acyl-CoA dehydrogenase medium chain contributes to the breakdown of medium-chain fatty acids to produce energy in cells.

Are there any known inhibitors or activators of this protein?

There are currently no known inhibitors or activators of acyl-CoA dehydrogenase medium chain protein.

What research is currently being conducted to further understand the function of acyl-CoA dehydrogenase medium chain protein?

Research is ongoing to further understand the function and regulation of acyl-CoA dehydrogenase medium chain protein, as well as its role in metabolic disorders.

What diseases or disorders are associated with mutations in this protein?

Mutations in this protein are associated with a variety of metabolic disorders, including medium-chain acyl-CoA dehydrogenase deficiency.

How is the expression of acyl-CoA dehydrogenase medium chain regulated?

The expression of acyl-CoA dehydrogenase medium chain is regulated by a variety of factors, including gene expression and protein degradation pathways.

Where is acyl-CoA dehydrogenase medium chain protein located within the cell?

Acyl-CoA dehydrogenase medium chain protein is located in the mitochondrial matrix.

What is the substrate specificity of this protein?

Acyl-CoA dehydrogenase medium chain protein has specificity for medium-chain fatty acyl-CoA substrates.

What is the structure of this protein and how does it relate to its function?

Acyl-CoA dehydrogenase medium chain protein has a flavin adenine dinucleotide (FAD)-binding domain that is essential for its function.

What is the function of acyl-CoA dehydrogenase medium chain protein?

Acyl-CoA dehydrogenase medium chain protein catalyzes the first step in the 尾-oxidation of medium-chain fatty acids.

What are the physiological roles of acyl-CoA dehydrogenase medium chain?

Acyl-CoA dehydrogenase medium chain plays a key role in energy metabolism and fatty acid oxidation in cells.