Recombinant Human ACAP1 293 Cell Lysate
Cat.No. : | ACAP1-9110HCL |
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Description : | Antigen standard for ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (ACAP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ACAP1 ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 [ Homo sapiens ] |
Official Symbol : | ACAP1 |
Synonyms : | ACAP1; ArfGAP with coiled-coil, ankyrin repeat and PH domains 1; centaurin, beta 1 , CENTB1; arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1; KIAA0050; cnt-b1; centaurin-beta-1; centaurin, beta 1; Arf GAP with coiled coil, ANK repeat and PH domains 1; CENTB1; |
Gene ID : | 9744 |
mRNA Refseq : | NM_014716 |
Protein Refseq : | NP_055531 |
MIM : | 607763 |
UniProt ID : | Q15027 |
Chromosome Location : | 17p13.1 |
Pathway : | Arf6 signaling events, organism-specific biosystem; Arf6 trafficking events, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; |
Function : | ARF GTPase activator activity; metal ion binding; phospholipase C activity; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
ACAP1-0001H | Recombinant Human ACAP1 Protein, GST-Tagged | +Inquiry |
Acap1-1487M | Recombinant Mouse Acap1 Protein, Myc/DDK-tagged | +Inquiry |
ACAP1-242M | Recombinant Mouse ACAP1 Protein, His (Fc)-Avi-tagged | +Inquiry |
ACAP1-3614B | Recombinant Bovine ACAP1, His-tagged | +Inquiry |
ACAP1-5076Z | Recombinant Zebrafish ACAP1 | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionRecombinant ACAP1 protein can be suitable for in vitro studies, such as biochemical assays or protein-protein interaction studies. However, it may not be suitable for in vivo studies, such as animal experiments, without appropriate modifications.
ACAP1 regulates actin cytoskeleton remodeling by interacting with Rho family GTPases, which are key regulators of the actin cytoskeleton. ACAP1 binds to the RhoA GTPase and promotes its activation, which leads to the formation of stress fibers and focal adhesions. ACAP1 also interacts with the Cdc42 GTPase and promotes the formation of filopodia and lamellipodia.
Creative BioMart offers customization options, such as the addition of tags or mutations. It is recommended to inquire with our scientist regarding customization options and the process involved. The addition of tags or mutations may affect the protein's function or analysis, and it is important to consider these factors when customizing the protein.
Recombinant ACAP1 protein is primarily used as a research tool and is not approved for diagnostic or therapeutic purposes. However, research on the function of ACAP1 may lead to the development of new diagnostic or therapeutic agents in the future.
It is often used as a positive control in Western blotting, ELISA, or other assays, or as a tool for studying the function of ACAP1.
ACAP1 has been shown to play a role in endocytic trafficking by regulating the formation of clathrin-coated vesicles. ACAP1 binds to the adaptor protein complex AP-2 and helps to recruit it to the plasma membrane. This promotes the formation of clathrin-coated vesicles, which are involved in the internalization of transmembrane receptors and other cargo molecules.
The expression system used for producing recombinant ACAP1 protein can vary depending on the specific product. Commonly used expression systems include E. coli, yeast, insect cells, and mammalian cells.
The specific buffer conditions or additives used when working with recombinant ACAN protein may depend on the specific experimental conditions and applications used. It is recommended to use buffers or media that are compatible with the protein's stability and solubility, and to avoid additives that may interfere with the protein's function or analysis. It is also important to consider the purity and endotoxin level of the protein, as these factors may affect its suitability for certain applications.
Yes, we can customize protein expression to meet your research requirements. Tagged versions can include histidine tags, GST tags, or other fusion partners that facilitate purification or detection of the protein.
Targeting ACAP1 may have potential clinical applications in cancer therapy. Inhibition of ACAP1 has been shown to reduce cancer cell migration and invasion in vitro and in vivo, suggesting that ACAP1 may be a promising target for the development of anti-cancer drugs. Additionally, ACAP1 may be a potential therapeutic target for other diseases that involve abnormal endocytic trafficking or actin cytoskeleton remodeling, such as neurodegenerative diseases and cardiovascular disorders.
Customer Reviews (3)
Write a reviewCreative BioMart's ability to customize recombinant proteins to meet my specific needs has been a game-changer for my research. I am grateful for their expertise and professionalism.
Creative BioMart's customer service is top-notch. They are always willing to go the extra mile to ensure that their customers are satisfied with their products.
I appreciate the collaboration I had with your scientists. They were able to create a custom recombinant protein that perfectly suited my needs.
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