Recombinant Human ACAP3, His-tagged

• Cat.No.: ACAP3-9275H
• Product Overview: Recombinant Human ACAP3 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Source: E.coli
• Species: Human
• Tag: His
• Protein length: C-term-348a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: ACAP3 ArfGAP with coiled-coil, ankyrin repeat and PH domains 3 [ Homo sapiens ]
• Official Symbol: ACAP3
• Synonyms: ACAP3; ArfGAP with coiled-coil, ankyrin repeat and PH domains 3; centaurin, beta 5 , CENTB5; arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 3; KIAA1716; cnt-b5; centaurin-beta-5; centaurin, beta 5; CENTB5
• Gene ID: 116983
• mRNA Refseq: NM_030649
• Protein Refseq: NP_085152
• UniProt ID: Q96P50
• Chromosome Location: 1p36
• Pathway: Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem
• Function: ARF GTPase activator activity; SH3 domain binding; cytoskeletal adaptor activity; metal ion binding; zinc ion binding

Reviews

08/22/2021

The recombinant human ACAP3 protein I purchased from Creative BioMart was easy to work with and highly effective. I highly recommend this product to others.

03/21/2021

I appreciate Creative BioMart's commitment to providing high-quality products at an affordable price. Their recombinant human ACAP3 protein is a great value for the money.

04/30/2020

The technical support team was extremely helpful in customizing the recombinant ACAP3 protein to my specific needs. The final product worked perfectly for my project.

Q&As

What is the recommended protein concentration for using the recombinant ACAP3 protein in experiments?

The recommended protein concentration for using the recombinant ACAP3 protein in experiments may vary depending on the specific experimental conditions, but a typical working concentration range is 0.1-10 μg/mL.

How is ACAP3 expression regulated in cells?

The expression of ACAP3 has been shown to be regulated by a variety of factors, including growth factors, cytokines, and cellular stress. For example, ACAP3 expression has been found to be upregulated in response to stimulation with epidermal growth factor (EGF) and platelet-derived growth factor (PDGF).

How does ACAP3 regulate exocytosis?

ACAP3 has been implicated in the regulation of exocytosis by interacting with proteins involved in vesicle fusion and secretion, such as the exocyst complex and the SNARE proteins. ACAP3's interaction with these proteins may help to coordinate the delivery of cargo to the plasma membrane and promote efficient secretion.

Can the recombinant ACAP3 protein be used for functional studies?

Yes, the recombinant ACAP3 protein can be used for functional studies, as it is a biologically active protein that is capable of binding to other proteins and performing its cellular functions.

Does the His tag of Recombinant Human ACAP3 (ACAP3-9275H) affect the activity of the protein?

The His tag promotes protein solubility and is used for affinity purification, it may or may not affect protein activity. If you are concerned that the tag may interfere with protein activity, Creative BioMart can also provide custom tag-free ACAP3 protein service.

Can the recombinant ACAP3 protein be used in diagnostic assays or therapeutic applications?

Recombinant ACAP3 protein is primarily used as a research tool and is not approved for diagnostic or therapeutic purposes.

I plan to buy ACAP3 protein, is there any discount for bulk?

Creative BioMart offers a bulk quote for each product available on our website. For specific prices, please contact our sales representatives.

Is there any additional processing or purification required before using the recombinant ACAP3 protein in experiments?

The recombinant ACAP3 protein is ready-to-use and does not require any additional processing or purification before use.

Can you provide detailed instructions for recombinant human ACAP3?

Yes, we offer MSDS files and data sheets with detailed product information.

What is the role of ACAP3 in endocytosis?

ACAP3 has been shown to regulate clathrin-mediated endocytosis by binding to clathrin and dynamin, two key proteins involved in this process. ACAP3's interaction with dynamin is thought to be important for the formation of endocytic vesicles, while its interaction with clathrin may modulate the timing and efficiency of vesicle formation.