Recombinant Human ACK1 protein(Gly110-Trp476), GST-tagged

• Cat.No.: ACK1-782H
• Product Overview: Recombinant Human ACK1(NP_005772.3)(Gly 110-Trp 476) was expressed in Insect Cells with the GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-GST
• Protein length: Gly110-Trp476
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 0.5mM EDTA, 0.5mM PMSF, 0.5mM TCEP.
• Bio-activity: The specific activity was determined to be ＞4 nmol/min/mg using synthetic Abl peptide (EAIYAAPFAKKK) as substrate.
• Molecular Mass: The recombinant human ACK1/GST chimera consists of 592 amino acids and predicts a molecular mass of 68 kDa. It migrates as an approximately 62 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.