Recombinant Human ACPL2 protein, His-tagged

• Cat.No.: ACPL2-0408H
• Product Overview: Recombinant Human ACPL2 protein(130-580 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 130-580 aa
• AA Sequence: EAFISHMSKGSGASFESPLNSLPLY PNHPLCEMGELTQTGVVQHLQNGQL LRDIYLKKHKLLPNDWSADQLYLET TGKSRTLQSGLALLYGFLPDFDWKK IYFRHQPSALFCSGSCYCPVRNQYL EKEQRRQYLLRLKNSQLEKTYGEMA KIVDVPTKQLRAANPIDSMLCHFCH NVSFPCTRNGCVDMEHFKVIKTHQI EDERERREKKLYFGYSLLGAHPILN QTIGRMQRATEGRKEELFALYSAHD VTLSPVLSALGLSEARFPRFAARLI FELWQDREKPSEHSVRILYNGVDVT FHTSFCQDHHKRSPKPMCPLENLVR FVKRDMFVALGGSGTNYYDACHREG F
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: ACPL2 acid phosphatase-like 2 [ Homo sapiens ]
• Official Symbol: ACPL2
• Synonyms: ACPL2; acid phosphatase-like 2; acid phosphatase-like protein 2; FLJ23751
• Gene ID: 92370
• mRNA Refseq: NM_001037172
• Protein Refseq: NP_001032249
• UniProt ID: Q8TE99

Reviews

04/05/2021

Just as they described on the site the package

03/20/2021

Good product.

12/10/2020

Workes great, very good product for a nice price.

07/31/2020

Fast delivery. Happy with purchase.

07/12/2018

This product is exactly as advertised with no issues.

Q&As

How does ACPL2 react with (+)-schisandrin B?

Schizandrin B inhibits the reaction [Carbon Tetrachloride results in decreased expression of PXYLP1 mRNA]

Could please list three molecular functions of ACPL2?

Lipid binding, oxidoreductase, and RNA binding.

How deos ACPL2 response to the phosphatase activity and acid phosphatase activity?

Responsible for the 2-O-dephosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature glycosaminoglycan (GAG) chains.

What kind of diseases associated with ACPL2 gene?

 Diseases associated with ACPL2 include Ehlers-Danlos Syndrome, Musculocontractural Type, 2 and Spondyloepimetaphyseal Dysplasia With Joint Laxity, Type 1, With Or Without Fractures.

What phenotypes occur when affected by ACPL2?

Body height, body fat distribution, and abnormality of refraction, etc.

What biological process (gene ontology) does ACPL2 gene involved?

They are glycosaminoglycan biosynthetic process, positive regulation of heparan sulfate proteoglycan biosynthetic process, dephosphorylation, and chondroitin sulfate proteoglycan biosynthetic process.

What is the bio-physicochemical properties of ACPL2 gene?

pH dependence: Optimum pH is 5.8.

How does ACPL2 influence the 1,2-dimethylhydrazine?

1, 2-Dimethylhydrazine results in decreased expression of PXYLP1 mRNA

How about he mRNA expression of ACPL2 gene?

Tissue specificity: Widely expressed. Strongly expressed in spleen, fetal liver, moderately in placenta, pancreas, kidney, thymus and colon.

Could please some protein tissue co-expression partners for ACPL2 gene?

C4orf19, TNFAIP1, XKR8, KCNJ2, KDF1, BMP5.