Recombinant Human ACSBG1 protein, His-tagged

• Cat.No.: ACSBG1-3948H
• Product Overview: Recombinant Human ACSBG1 protein(404-686 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein Length: 404-686 aa
• AA Sequence: AMSVTLEQNLTCPGSDLKPFTTRLA DYLVLAKVRQALGFAKCQKNFYGAA PMMAETQHFFLGLNIRLYAGYGLSE TSGPHFMSSPYNYRLYSSGKLVPGC RVKLVNQDAEGIGEICLWGRTIFMG YLNMEDKTCEAIDEEGWLHTGDAGR LDADGFLYITGRLKELIITAGGENV PPVPIEEAVKMELPIISNAMLIGDQ RKFLSMLLTLKCTLDPDTSDQTDNL TEQAVEFCQRVGSRATTVSEIIEKK DEAVYQAIEEGIRRVNMNAAARPYH IQKWAILE
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: ACSBG1 acyl-CoA synthetase bubblegum family member 1 [ Homo sapiens ]
• Official Symbol: ACSBG1
• Synonyms: ACSBG1; acyl-CoA synthetase bubblegum family member 1; long-chain-fatty-acid--CoA ligase ACSBG1; BG1; BGM; bubblegum; FLJ30320; hBG1; hsBG; KIAA0631; lipidosin; MGC14352; very long chain acyl CoA synthetase; very long-chain acyl-CoA synthetase; BG; LPD; GR-LACS
• Gene ID: 23205
• mRNA Refseq: NM_001199377
• Protein Refseq: NP_001186306
• MIM: 614362
• UniProt ID: Q96GR2

Reviews

07/19/2020

The samples provided by the manufacturer allowed me to test out different proteins before committing to a larger purchase, which was extremely helpful.

04/23/2020

The manufacturer's consistency in delivering high quality proteins has made them my go-to supplier.

08/16/2019

I was impressed by the follow-up from the manufacturer's after-sales team, who ensured that I was satisfied with my purchase.

07/09/2019

A satisfying purchase

07/13/2018

All good. Will recommand to my other colleague

Q&As

What is the relationship between ACSBG1 and insulin resistance?

ACSBG1 knockout in animal models leads to improved insulin sensitivity, and ACSBG1 expression is upregulated in insulin-resistant states.

What is the primary tissue distribution of ACSBG1 expression?

ACSBG1 is primarily expressed in the liver and adipose tissue, but is also found in other tissues such as the brain.

What is the role of ACSBG1 in insulin signaling?

ACSBG1 is involved in insulin signaling by regulating the levels of diacylglycerols, which can activate PKCε and inhibit insulin signaling.

What is the mechanism of ACSBG1 regulation by PGC-1α?

PGC-1α regulates ACSBG1 expression by interacting with PPARα to increase transcription, as well as by promoting the acetylation of histones in the ACSBG1 promoter region.

What is the role of ACSBG1 in cancer?

ACSBG1 has been found to be upregulated in several types of cancer, including breast, prostate, and liver cancer, and may play a role in tumor growth and progression.

What is the role of ACSBG1 in lipid droplet formation?

ACSBG1 is involved in the formation of lipid droplets by promoting the esterification of fatty acids into triacylglycerols.

How is the expression of ACSBG1 regulated?

ACSBG1 expression is regulated by a number of transcription factors, including SREBP1c, PPARα, and PGC-1α, which are all involved in regulating lipid metabolism.

What is the role of ACSBG1 in non-alcoholic fatty liver disease (NAFLD)?

ACSBG1 expression is upregulated in the liver of individuals with NAFLD, and ACSBG1 knockout in animal models leads to reduced hepatic steatosis.

What is the mechanism of ACSBG1 regulation by PPARα?

PPARα regulates ACSBG1 expression by binding to a specific site in the ACSBG1 promoter and increasing transcription.

What is the substrate specificity of ACSBG1?

ACSBG1 has a broad substrate specificity and can activate a variety of long-chain fatty acids.