Recombinant Human ACSL4 293 Cell Lysate
Cat.No. : | ACSL4-9075HCL |
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Description : | Antigen standard for acyl-CoA synthetase long-chain family member 4 (ACSL4), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC221413, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ACSL4 acyl-CoA synthetase long-chain family member 4 [ Homo sapiens ] |
Official Symbol : | ACSL4 |
Synonyms : | ACSL4; acyl-CoA synthetase long-chain family member 4; FACL4, fatty acid Coenzyme A ligase, long chain 4 , mental retardation, X linked 63 , mental retardation, X linked 68 , MRX63, MRX68; long-chain-fatty-acid--CoA ligase 4; long chain fatty acid Coenzyme A ligase 4; ACS4; LACS4; lignoceroyl CoA synthase; LACS 4; acyl-CoA synthetase 4; lignoceroyl-CoA synthase; long-chain acyl-CoA synthetase 4; long-chain fatty-acid-Coenzyme A ligase 4; fatty-acid-Coenzyme A ligase, long-chain 4; FACL4; MRX63; MRX68; |
Gene ID : | 2182 |
mRNA Refseq : | NM_022977 |
Protein Refseq : | NP_075266 |
MIM : | 300157 |
UniProt ID : | O60488 |
Chromosome Location : | Xq22.3-q23 |
Pathway : | Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Fatty Acid Beta Oxidation, organism-specific biosystem; Fatty Acid Biosynthesis, organism-specific biosystem; Fatty Acyl-CoA Biosynthesis, organism-specific biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem; |
Function : | ATP binding; arachidonate-CoA ligase activity; ligase activity; long-chain fatty acid-CoA ligase activity; nucleotide binding; |
Products Types
◆ Recombinant Protein | ||
ACSL4-55H | Recombinant Human ACSL4 Protein, His-tagged | +Inquiry |
ACSL4-46R | Recombinant Rhesus Macaque ACSL4 Protein, His (Fc)-Avi-tagged | +Inquiry |
ACSL4-127R | Recombinant Rat ACSL4 Protein, His (Fc)-Avi-tagged | +Inquiry |
Acsl4-56R | Recombinant Rat Acsl4 Protein, His-tagged | +Inquiry |
Acsl4-1507M | Recombinant Mouse Acsl4 Protein, Myc/DDK-tagged | +Inquiry |
◆ Lysates | ||
ACSL4-9074HCL | Recombinant Human ACSL4 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionACSL4 has been shown to impact gene expression by regulating the availability of long-chain fatty acids for the production of signaling molecules that can impact gene expression.
ACSL4 mutations have been linked to a variety of human diseases, including metabolic disorders, neurodegenerative diseases, and cancer.
ACSL4 dysregulation has been linked to the development of neurodegenerative diseases, such as Alzheimer's disease and Parkinson's disease, as it can contribute to alterations in lipid metabolism and oxidative stress.
ACSL4 has been shown to impact oxidative stress, a condition characterized by an imbalance between the production of reactive oxygen species and antioxidant defenses, by regulating the availability of long-chain fatty acids for the production of antioxidant molecules.
ACSL4 has been shown to impact mitochondrial function by regulating the availability of long-chain fatty acids for energy production and cellular signaling pathways.
ACSL4 dysregulation has been implicated in the development of cancer, as it can promote tumor cell survival and growth by altering lipid metabolism and signaling pathways.
ACSL4 has been shown to play a role in inflammation by regulating the availability of long-chain fatty acids for the production of inflammatory mediators, such as prostaglandins and leukotrienes.
ACSL4 dysregulation has been linked to the development of insulin resistance, a condition characterized by impaired glucose uptake and utilization, which can contribute to the development of metabolic disorders, such as obesity and diabetes.
ACSL4 expression is regulated by a variety of mechanisms, including transcriptional regulation, post-transcriptional regulation, and post-translational modifications.
The ACSL4 gene is expressed in a variety of tissues, including adipose tissue, liver, muscle, heart, and brain.
Customer Reviews (5)
Write a reviewHigh quality product I got from here!
A+ for this recombinant protein. I don't think I've ever been this excited about a scientific purchase before.
I'm pretty sure this protein is giving me superpowers. Okay, maybe not, but it's definitely awesome.
I don't know how you did it, but this protein is like liquid gold. Keep up the good work!
Can I just say how much I love this company? The protein is great, but the vibes are even better.
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