Recombinant Rat Acsl4 Protein, His-tagged

• Cat.No.: Acsl4-56R
• Product Overview: Recombinant Rat Acsl4 Protein (Lys459-Lys670) with N-His tag was expressed in E. coli.

Specification

• Description: Enables arachidonate-CoA ligase activity. Involved in several processes, including dendritic spine development; long-chain fatty-acyl-CoA biosynthetic process; and positive regulation of insulin secretion. Located in mitochondrial membrane; neuronal cell body; and peroxisome. Colocalizes with endoplasmic reticulum. Human ortholog(s) of this gene implicated in intellectual disability and non-syndromic X-linked intellectual disability 63. Orthologous to human ACSL4 (acyl-CoA synthetase long chain family member 4).
• Source: E. coli
• Species: Rat
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 27.7 kDa
  Accurate Molecular Mass: 28 kDa
• Protein length: Lys459-Lys670
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Acsl4 acyl-CoA synthetase long-chain family member 4 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Acsl4
• Synonyms: ACSL4; acyl-CoA synthetase long-chain family member 4; long-chain-fatty-acid--CoA ligase 4; LACS 4; long-chain acyl-CoA synthetase 4; fatty acid-Coenzyme A ligase long chain 4; fatty acid Coenzyme A ligase, long chain 4; fatty acid-Coenzyme A ligase, long chain 4; Acs4; Facl4
• Gene ID: 113976
• mRNA Refseq: NM_053623
• Protein Refseq: NP_446075
• UniProt ID: O35547

Reviews

06/18/2021

High quality product I got from here!

02/23/2020

A+ for this recombinant protein. I don't think I've ever been this excited about a scientific purchase before.

02/01/2020

I'm pretty sure this protein is giving me superpowers. Okay, maybe not, but it's definitely awesome.

11/04/2019

I don't know how you did it, but this protein is like liquid gold. Keep up the good work!

01/18/2019

Can I just say how much I love this company? The protein is great, but the vibes are even better.

Q&As

What is the significance of ACSL4 in the regulation of gene expression?

ACSL4 has been shown to impact gene expression by regulating the availability of long-chain fatty acids for the production of signaling molecules that can impact gene expression.

What is the impact of ACSL4 mutations on human health?

ACSL4 mutations have been linked to a variety of human diseases, including metabolic disorders, neurodegenerative diseases, and cancer.

What is the impact of ACSL4 dysregulation on neurodegenerative diseases?

ACSL4 dysregulation has been linked to the development of neurodegenerative diseases, such as Alzheimer's disease and Parkinson's disease, as it can contribute to alterations in lipid metabolism and oxidative stress.

What is the relationship between ACSL4 and oxidative stress?

ACSL4 has been shown to impact oxidative stress, a condition characterized by an imbalance between the production of reactive oxygen species and antioxidant defenses, by regulating the availability of long-chain fatty acids for the production of antioxidant molecules.

What is the role of ACSL4 in mitochondrial function?

ACSL4 has been shown to impact mitochondrial function by regulating the availability of long-chain fatty acids for energy production and cellular signaling pathways.

What is the significance of ACSL4 in the development of cancer?

ACSL4 dysregulation has been implicated in the development of cancer, as it can promote tumor cell survival and growth by altering lipid metabolism and signaling pathways.

What is the relationship between ACSL4 and inflammation?

ACSL4 has been shown to play a role in inflammation by regulating the availability of long-chain fatty acids for the production of inflammatory mediators, such as prostaglandins and leukotrienes.

What is the impact of ACSL4 dysregulation on insulin sensitivity?

ACSL4 dysregulation has been linked to the development of insulin resistance, a condition characterized by impaired glucose uptake and utilization, which can contribute to the development of metabolic disorders, such as obesity and diabetes.

What are the regulatory mechanisms that control ACSL4 expression?

ACSL4 expression is regulated by a variety of mechanisms, including transcriptional regulation, post-transcriptional regulation, and post-translational modifications.

What is the expression pattern of the ACSL4 gene?

The ACSL4 gene is expressed in a variety of tissues, including adipose tissue, liver, muscle, heart, and brain.