Recombinant Human ACSM1 293 Cell Lysate

• Cat.No.: ACSM1-9072HCL

Specification

• Description: Antigen standard for acyl-CoA synthetase medium-chain family member 1 (ACSM1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ACSM1 acyl-CoA synthetase medium-chain family member 1 [ Homo sapiens ]
• Official Symbol: ACSM1
• Synonyms: BUCS1; MACS1
• Gene ID: 116285
• mRNA Refseq: NM_052956.2
• Protein Refseq: NP_443188.2
• MIM: 614357
• UniProt ID: Q08AH1
• Chromosome Location: 16p12.3
• Pathway: Amino Acid conjugation, organism-specific biosystem;Biological oxidations, organism-specific biosystem;Butanoate metabolism, organism-specific biosystem;Butanoate metabolism, conserved biosystem;Conjugation of benzoate with glycine, organism-specific biosystem;Conjugation of carboxylic acids, organism-specific biosystem;Conjugation of phenylacetate with glutamine, organism-specific biosystem
• Function: ATP binding;GTP binding;acyl-CoA ligase activity;butyrate-CoA ligase activity;fatty acid ligase activity

Reviews

01/04/2022

It performs exceptionally well in a variety of experimental applications, proving its broad utility.

08/13/2021

The price was competitive and fair, especially considering the high quality of the protein.

04/29/2021

The performance of this protein in my lab has been exceptional. It consistently yields robust and reproducible results, making it an invaluable tool for our research. Highly recommended!

12/04/2019

The protein's bioactivity is impressive. It induces the desired cellular response in our functional assays, proving its efficacy in our research.

07/03/2019

Budget-friendly protein without compromising on quality. Service was exceptional too.

Q&As

What is the role of ACSM1 in the gut microbiome?

Recent studies have shown that ACSM1 expression in the gut is associated with changes in the gut microbiome and may play a role in regulating the gut-brain axis.

How does ACSM1 expression change in response to exercise?

ACSM1 expression has been shown to increase in response to exercise, which may help to increase the utilization of medium-chain fatty acids for energy.

How does ACSM1 activity affect exercise performance?

ACSM1 activity has been shown to be important for the utilization of medium-chain fatty acids during exercise and may contribute to improved exercise performance.

How is ACSM1 expression regulated by circadian rhythms?

ACSM1 expression is regulated by the circadian clock and may play a role in the regulation of metabolic processes.

What is the potential therapeutic value of targeting ACSM1?

ACSM1 inhibition has been suggested as a potential therapeutic strategy for the treatment of metabolic disorders such as obesity, type 2 diabetes, and nonalcoholic fatty liver disease.

What post-translational modifications can affect ACSM1 protein function?

Phosphorylation and acetylation have been shown to affect ACSM1 enzyme activity and may play a role in regulating fatty acid metabolism.

How does ACSM1 contribute to the development of breast cancer?

Recent studies have shown that ACSM1 expression is increased in breast cancer cells and may play a role in promoting tumor growth and metastasis.

How does ACSM1 contribute to the development of nonalcoholic fatty liver disease (NAFLD)?

Recent studies have shown that ACSM1 expression is increased in the liver of patients with NAFLD and may contribute to the development of this condition by promoting hepatic lipid accumulation.

How is ACSM1 expression regulated by epigenetic modifications?

Recent studies have shown that DNA methylation and histone modifications can regulate ACSM1 gene expression, potentially playing a role in the development of metabolic disorders.

What is the role of ACSM1 in brown adipose tissue (BAT) function?

Recent studies have shown that ACSM1 expression in BAT is associated with increased thermogenesis and energy expenditure.