Recombinant Human ACSS3 293 Cell Lysate
Cat.No. : | ACSS3-9067HCL |
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Description : | Antigen standard for acyl-CoA synthetase short-chain family member 3 (ACSS3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ACSS3 acyl-CoA synthetase short-chain family member 3 [ Homo sapiens ] |
Official Symbol : | ACSS3 |
Synonyms : | ACSS3; acyl-CoA synthetase short-chain family member 3; acyl-CoA synthetase short-chain family member 3, mitochondrial; FLJ21963; AMP-binding enzyme, 33217; |
Gene ID : | 79611 |
mRNA Refseq : | NM_024560 |
Protein Refseq : | NP_078836 |
MIM : | 614356 |
UniProt ID : | Q9H6R3 |
Chromosome Location : | 12q21.31 |
Pathway : | Metabolic pathways, organism-specific biosystem; Propanoate metabolism, organism-specific biosystem; Propanoate metabolism, conserved biosystem; acetate conversion to acetyl-CoA, organism-specific biosystem; ethanol degradation II, organism-specific biosystem; ethanol degradation IV, organism-specific biosystem; oxidative ethanol degradation III, organism-specific biosystem; |
Function : | ATP binding; acetate-CoA ligase activity; ligase activity; nucleotide binding; |
Products Types
◆ Recombinant Protein | ||
ACSS3-280M | Recombinant Mouse ACSS3 Protein, His (Fc)-Avi-tagged | +Inquiry |
ACSS3-265H | Recombinant Human ACSS3 Protein, His (Fc)-Avi-tagged | +Inquiry |
Acss3-1514M | Recombinant Mouse Acss3 Protein, Myc/DDK-tagged | +Inquiry |
ACSS3-18H | Recombinant Human ACSS3, His-tagged | +Inquiry |
ACSS3-211H | Recombinant Human ACSS3 Protein, GST-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionACSS3 has been found to be upregulated in certain cancers and is associated with tumor growth, progression, and metabolic reprogramming.
Yes, ACSS3 can influence gene expression through its role in protein acetylation, which can modify histones and transcription factors.
ACSS3 activity is sensitive to cellular energy levels, particularly levels of ATP (adenosine triphosphate), which is an indicator of energy availability.
ACSS3 expression can be regulated by various factors, including nutrient availability, cellular energy status, and transcriptional control.
Acetyl-CoA is involved in various metabolic processes, including the citric acid cycle (Krebs cycle), fatty acid synthesis, and protein acetylation.
Recent studies have linked dysregulation of ACSS3 to various diseases, including cancer, obesity, and metabolic disorders.
Yes, there are commercially available antibodies and kits that can be used for detecting ACSS3 protein in biological samples, such as Western blotting or immunohistochemistry.
Yes, ACSS3 protein can be produced using recombinant DNA technology by cloning the ACSS3 gene into an expression vector and expressing it in a suitable host organism, such as bacteria or yeast.
The activity of ACSS3 can be measured through enzymatic assays that assess the conversion of acetate to acetyl-CoA in vitro, using purified ACSS3 protein or cell lysates.
Common techniques for purifying ACSS3 protein include affinity chromatography, size exclusion chromatography, and ion exchange chromatography.
Customer Reviews (5)
Write a reviewUnbeatable price for the exceptional quality of the protein. Service was impeccable.
Outstanding quality at an affordable price. Impressed with the value for money.
Versatile and compatible. Fits various experimental setups."
"The performance of this protein is truly remarkable. Its consistent and reliable results have significantly contributed to the success of our research endeavors.
It eliminates background noise and provides clear and interpretable data.
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