Recombinant Human ADAM10 Protein, GST-tagged

• Cat.No.: ADAM10-272H
• Product Overview: Human ADAM10 partial ORF ( NP_001101, 346 a.a. - 435 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. [provided by RefSeq, Feb 2016]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 35.64 kDa
• AA Sequence: KSKLYSDGKKKSLNTGIITVQNYGS HVPPKVSHITFAHEVGHNFGSPHDS GTECTPGESKNLGQKENGNYIMYAR ATSGDKLNNNKFSLC
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ADAM10 ADAM metallopeptidase domain 10 [ Homo sapiens ]
• Official Symbol: ADAM10
• Synonyms: ADAM10; ADAM metallopeptidase domain 10; a disintegrin and metalloproteinase domain 10; disintegrin and metalloproteinase domain-containing protein 10; CD156c; HsT18717; kuz; MADM; CDw156; ADAM 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10; AD10
• Gene ID: 102
• mRNA Refseq: NM_001110
• Protein Refseq: NP_001101
• MIM: 602192
• UniProt ID: O14672

Reviews

09/16/2021

During the experiment, the technician helped me solve the problems in a timely and accurate manner.

04/03/2021

The ADAM10 proteins purchased from your company is suitable for WB.

09/08/2020

Received high quality protein products and obtained satisfactory experimental results.

Q&As

What proteins does ADAM10 cleave?

ADAM10 protein can cleave TNF-α and E-cadherin, etc.

Does adding GST tag have any effect on protein?

The addition of the GST tag helps protect the recombinant protein from extracellular protease degradation and improves its stability.

How to distinguish between ADAM10 precursors and active bodies?

The protein size of ADAM10 precursor is larger than that of ADAM10 active.

In addition to cardiovascular tumor-related research, ADAM10 is currently used in the research of which diseases?

ADAD1 is currently also used in the research of Alzheimer's disease and diabetic retinopathy, etc.