Recombinant Human ADAM12 protein, His-tagged

• Cat.No.: ADAM12-365H
• Product Overview: Recombinant Human ADAM12 protein (Arg29-Asp513), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: A Disintegrin And Metalloprotease 12 (ADAM12) is also known as Meltrin-alpha and MCMP, is a member of the ADAM and MDC (Metalloprotease, Disintegrin, Cysteine-rich) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. Over 30 members have been identified and about half of them are active metalloproteases such as ADAM8, 9, 10, 12 and 17/TACE. ADAM12 has four Isoform, Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. ADAM12 Involved in skeletal muscle regeneration, specifically at the onset of cell fusion and also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 55.2 kDa (Propeptide 20.1 kDa and mature form 34.5 kDa). Human ADAM12, His Tag was cleaved into the propeptide and the mature form. The reducing (R) protein migrates as 65 kDa (Propeptide 23 kDa and mature form 46 kDa) in SDS-PAGE due to glycosylation, corresponding to the proprotein form, the mature form and the propeptide respectively.
• Protein length: 485
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: ADAM12
• Official Symbol: ADAM12
• Synonyms: ADAM12; ADAM metallopeptidase domain 12; a disintegrin and metalloproteinase domain 12 (meltrin alpha); disintegrin and metalloproteinase domain-containing protein 12; MCMPMltna; meltrin alpha; MLTN; meltrin-alpha; MCMP; MLTNA
• Gene ID: 8038
• mRNA Refseq: NM_003474
• Protein Refseq: NP_003465
• MIM: 602714
• UniProt ID: O43184

Reviews

08/22/2020

The technical staff evaluated my experimental process and results and made many constructive comments

05/28/2019

Protein products are put into experiments with good effect and reliable experimental results

Q&As

In addition to studies related to breast cancer, what other diseases is ADAD12 currently being used to study?

Currently, ADAM12 is also involved in the study of lung cancer and liver cancer.

Does ADAM12 affect Vimentin expression?

ADAM12 upregulates the expression of Vimentin.

Does ADAM12 interact with insulin-like growth factor-binding protein-3 (IGFBP)-3?

ADAM12 interacts with insulin-like growth factor binding protein(IGFBP)-3.

I want to buy ADAM12-related proteins, do you provide other proteins?

We offer myoferlin and Vimentin proteins in addition to ADAM12 proteins.

What protein does ADAM12 hydrolyze?

ADAM12 protein can hydrolyze Delta-like 1、Ephrin-A1, etc.

What are the two conformations of ADAM12?

The two conformations of ADAM are the membrane-penetrating full-length type (ADAM12-L) and the secretory type (ADAM12-S), respectively.