Recombinant Human ADAM19 293 Cell Lysate

• Cat.No.: ADAM19-9038HCL

Specification

• Description: Antigen standard for ADAM metallopeptidase domain 19 (meltrin beta) (ADAM19) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADAM19 ADAM metallopeptidase domain 19 [ Homo sapiens ]
• Official Symbol: ADAM19
• Synonyms: ADAM19; ADAM metallopeptidase domain 19; a disintegrin and metalloproteinase domain 19 (meltrin beta); disintegrin and metalloproteinase domain-containing protein 19; meltrin beta; MLTNB; ADAM 19; meltrin-beta; metalloprotease and disintegrin dendritic antigen marker; FKSG34; MADDAM
• Gene ID: 8728
• mRNA Refseq: NM_033274
• Protein Refseq: NP_150377
• MIM: 603640
• UniProt ID: Q9H013
• Chromosome Location: 5q33.3
• Function: SH3 domain binding; metal ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding

Reviews

06/13/2021

I have purchased protein products that meet my requirements. Although the customization time will be longer, the experimental results are very good.

01/17/2020

I just caught up with the promotional offer and got a satisfying protein at an affordable price.

05/05/2019

The product parameters are consistent with the information provided before, and the technicians are always able to answer my questions in a timely manner

Q&As

What are the domains of ADAM19?

Prodomain, metalloprotease domain, disintegrin domain, cysteine-rich domain, EGF-like domain, transmembrane domain, and cytoplasmic tail.

Where is the cellular localization of ADAM19?

ADAM19 is located in the Golgi apparatus.

Do you offer ADAM19 protein with other tags?

Yes, you can communicate specific requirements with our experts.

Can the endotoxin in ADAM19 protein be removed?

We can remove endotoxins according to your needs.

Can recombinant ADAM19 protein be obtained by other expression systems?

Yes, we provide bacterial expression systems, baculovirus-insect cell expression systems, cell-free in vitro protein expression systems, mammalian expression systems, yeast expression systems and other expression systems.

Is ADAM19 widely used in disease research?

ADAM19 has been used in related studies such as tumors, inflammatory diseases, and cardiovascular diseases.

Can I use PVDF membranes for western blotting of ADAM19?

Western blotting of ADAM19 can be performed using PVDF membranes.

In which tissues is ADAM19 highly expressed?

ADAM19 is expressed at high levels in bone, heart and lung.

Is this product a lyophilized powder or a liquid?

This depends on stock availability, or you can tell us your preference.

Can you give me some advice on ADAM19 antibody selection?

Yes, you have direct access to our experts who can provide advice tailored to your specific situation.

Do you only offer this one ADAM19 product?

ADAM19's spot product is currently this one, but we can provide customized production of other ADAM19.

Does ADAM19 have α-secretase activity on amyloid precursor protein?

ADAM19 has constitutive α-secretase activity for amyloid precursor protein.

At what pH is ADAM19 most active?

ADAM19 is more active at basic pH.

Do you provide a western blot protocol for ADAM19 along with the product?

You can make this request with our staff at the time of purchase.