Active Recombinant Human ADAM28 protein, His-tagged

• Cat.No.: ADAM28-212H
• Product Overview: Recombinant Human ADAM28(Leu72 & Leu191-Ala623) fused with His tag at C-terminal was expressed in CHO.

Specification

• Description: A distintegrin and metalloprotease domain-containing protein 28 (ADAM28), also known as MDC-L, is a member of the M12B peptidase family of enzymes. It is synthesized as an approximately 80-90 kDa glycosylated proprotein that is processed to a mature form later in the secretory pathway. After the removal of the propeptide that contains a cysteine switch motif, the activated form of ADAM28 consists of a 467 aa extracellular domain (ECD) which contains a peptidase, disintegrin, cysteine-rich and EGF-like domains, followed by a 21 aa transmembrane segment and an 89 aa cytoplasmic domain. Alternative splicing generates additional isoforms with a variety of substitutions and deletions in the cysteine-rich and EGF-like domains. Within the ECD, human ADAM28 shares 73% aa sequence identity with mouse and rat ADAM28. ADAM28 is a cell surface protein that is involved in a variety of cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis, and exhibits catalytic activity to insulin-like growth factor binding protein-3. Like many members of the ADAM proteins, the disintegrin domain of ADAM28 interacts with integrin to influence cell adhesion and cell-cell interaction. ADAM28 can mediate the adhesion of the Jurkat cells, T-lymphoma cell line, through integrin alpha 4b1(9). R&D Systems in-house testing also indicates that ADAM28 cleaves IGFBP-3.
• Source: CHO
• Species: Human
• Tag: His
• Predicted N Terminal: Leu72 & Leu191
• Form: Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
• Bio-activity: Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.2-1 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 49 & 62 kDa
  SDS-PAGE: 59-80 kDa, reducing conditions
• Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
• Purity: >70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 200 μg/mL in water.

Gene Information

• Gene Name: ADAM28 ADAM metallopeptidase domain 28 [ Homo sapiens ]
• Official Symbol: ADAM28
• Synonyms: ADAM28; ADAM metallopeptidase domain 28; a disintegrin and metalloproteinase domain 28; disintegrin and metalloproteinase domain-containing protein 28; ADAM23; eMDCII; MDC Lm; MDC Ls; metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L; epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich protein II; MDCL; MDC-L; MDC-Lm; MDC-Ls; ADAM 28; eMDC II
• Gene ID: 10863
• mRNA Refseq: NM_014265
• Protein Refseq: NP_055080
• MIM: 606188
• UniProt ID: Q9UKQ2