Recombinant Human ADAM30 293 Cell Lysate

• Cat.No.: ADAM30-9035HCL

Specification

• Description: Antigen standard for ADAM metallopeptidase domain 30 (ADAM30) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADAM30 ADAM metallopeptidase domain 30 [ Homo sapiens ]
• Official Symbol: ADAM30
• Synonyms: ADAM30; ADAM metallopeptidase domain 30; a disintegrin and metalloproteinase domain 30; disintegrin and metalloproteinase domain-containing protein 30; svph4
• Gene ID: 11085
• mRNA Refseq: NM_021794
• Protein Refseq: NP_068566
• MIM: 604779
• UniProt ID: Q9UKF2
• Chromosome Location: 1p13-p11
• Function: metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding

Reviews

11/05/2022

High protein purity, good experimental effect

06/30/2020

ADAM30 proteins purchased are as advertised and the quality of the product is guaranteed.

06/06/2020

The technician gave me a thorough introduction to the ADAM30 protein so that I could understand its biochemical properties.

02/08/2020

Excellent after-sales service, solving many experimental problems.

Q&As

Are there any other options for protein expression systems if I have a custom need?

We have established a comprehensive protein expression platform with several protein expression systems platforms, including bacterial expression systems, baculovirus-insect cell expression systems, cell-free in vitro protein expression systems, mammalian expression systems, yeast expression systems, etc.

Is it possible to add a tag other than HIS and GST?

Yes, we can customize the production of proteins according to your needs.

Can ADAM30 be used in neurological disease research?

ADAM30 has been used in research on Alzheimer's disease.

I want to buy ADAM28 for ELISA, which product is more suitable?

You can talk directly with our experts who will be able to give you useful advice based on your study.

Can I customize my ADAM30 with a C-terminal tag?

Yes, you can talk directly to our experts about your specific customization requirements.

If I have special customization needs, can I participate in this promotion?

Yes, you can participate in this promotion.

Can the GST tag of ADAM30 be removed?

Yes, we can customize the protein according to your needs.

If I have a custom request other than the optional service below, do you still accept customization?

You can talk directly to our experts about your specific customization requirements.

What physiological processes is ADAM30 involved in?

ADAM30 is involved in physiological processes such as fertilization, muscle development and neurogenesis.

Is it possible to adjust the purity level of the protein according to my requirements?

Yes, we can customize the purity level of the proteins according to your needs.

Can you provide ADAM30 expressed by different expression systems?

We have a wide range of expression systems such as bacterial expression systems, cell-free in vitro protein expression systems, mammalian expression systems, yeast expression systems, etc., which can be selected according to your needs.

Can the His tag be replaced with another one?

Yes,we will add tags according to your needs.

What methods do you use for protein purification?

We usually purify proteins by hydrophobic chromatography, affinity chromatography, ion exchange chromatography, salting out, molecular sieve, etc.