Recombinant Human ADAM32 293 Cell Lysate

• Cat.No.: ADAM32-9034HCL

Specification

• Description: Antigen standard for ADAM metallopeptidase domain 32 (ADAM32) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADAM32 ADAM metallopeptidase domain 32 [ Homo sapiens ]
• Official Symbol: ADAM32
• Synonyms: ADAM32; ADAM metallopeptidase domain 32; a disintegrin and metalloproteinase domain 32; disintegrin and metalloproteinase domain-containing protein 32; ADAM 32; metalloproteinase 12-like protein; a disintegrin and metalloprotease domain 32; FLJ26299; FLJ29004
• Gene ID: 203102
• mRNA Refseq: NM_145004
• Protein Refseq: NP_659441
• UniProt ID: Q8TC27
• Chromosome Location: 8p11.22
• Function: metal ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding

Reviews

10/21/2022

The ADAM32 I purchased was used in WB with very satisfactory results.

08/19/2022

The shipment was fast, and satisfactory experimental results were obtained.

06/09/2021

The product brochure is very detailed and has comprehensive basic information about the ADAM32 protein.

07/03/2020

The staff actively communicated with me and solved many technical problems.

Q&As

Does adding His tag have any effect on protein?

Addition of His tags usually does not affect the function, properties of the target protein

I need to buy Adam23 protein with other tags, do you offer them?

We are able to customize the protein to meet your needs.

I urgently need to buy adam32, how soon can you ship it?

We will arrange shipment as soon as possible after your purchase.

What proteins does ADAM32 have higher homology with?

ADAM32 is highly homologous to ADAM2 and ADAM3

Do you offer higher purity ADAM32?

We offer custom production of recombinant proteins and can provide higher purity ADAM32 protein.

Is it possible to combine purchases of different types of proteins to get more promotional discounts?

You can first communicate your purchase requirements directly with us, this is related to the quantity you buy.

What tag is usually connected to ADAM32?

Generally, His or GST is added, and we can also add appropriate tags according to your needs.

I need a custom endotoxin removal ADAM32 protein, can you meet this requirement?

Yes, we can custom produce ADAM32 protein according to your requirements.

In which tissues is ADAM32 highly expressed?

ADAM32 is mainly expressed in the testis

How do you ship ADAM32 with two different product forms?

We will ship based on stock availability.

I want to buy adam32 for western blotting, do you provide the corresponding antibodies?

We do not offer any ADAM32-related antibodies at this time.

Can I get additional discounts or offers if I purchase multiple custom protein products?

This depends on the quantity you purchase.

What are the functions of ADAM32?

ADAM32 has the function of regulating cell proliferation and migration, and can be further involved in the process of apoptosis

In which disease studies is ADAM32 used?

ADAM32 used in research on liver cancer, infertility and other diseases