Recombinant Human ADAM33 293 Cell Lysate

• Cat.No.: ADAM33-9033HCL

Specification

• Description: Antigen standard for ADAM metallopeptidase domain 33 (ADAM33), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADAM33 ADAM metallopeptidase domain 33 [ Homo sapiens ]
• Official Symbol: ADAM33
• Synonyms: ADAM33; ADAM metallopeptidase domain 33; a disintegrin and metalloproteinase domain 33 , C20orf153, chromosome 20 open reading frame 153; disintegrin and metalloproteinase domain-containing protein 33; dJ964F7.1; DKFZp434K0521; ADAM 33; a disintegrin and metalloprotease 33; a disintegrin and metalloproteinase domain 33; disintegrin and reprolysin metalloproteinase family protein; C20orf153; DJ964F7.1; FLJ35308; FLJ36751; MGC71889; MGC149823
• Gene ID: 80332
• mRNA Refseq: NM_025220
• Protein Refseq: NP_079496
• MIM: 607114
• UniProt ID: Q9BZ11
• Chromosome Location: 20p13
• Function: metal ion binding; metalloendopeptidase activity; metalloendopeptidase activity; peptidase activity; zinc ion binding; zinc ion binding

Reviews

12/26/2021

Very professional customer service, fully understanding the needs of the customer and giving professional advice.

09/14/2021

The purchased ADAM33 protein is suitable for WB experiments and I am satisfied with the results.

03/27/2021

The after-sales service is very thoughtful and helped me solve many technical problems satisfactorily.

Q&As

What is the main focus of ADAM33's current research?

ADAM33 current research focuses on asthma

I want to buy ADAM33 protein for WB, which membrane should I choose?

You can choose PVDF membrane.

I want to buy ADAM33 for ELISA, which product should I choose?

The choice will depend on your specific research needs and you can talk directly with our experts.

Does the addition of GST tag have any effect on the function of ADAM33 protein and downstream experiments?

The large molecular weight of GST tag may affect the protein function and downstream experiments.

What are the functions of ADAM33 protein?

ADAM33 protein has cell activation, protein hydrolysis and signaling functions

Does ADAM33 bind to transcription factor GATA3?

Transcription factor GATA3 binds to the core promoter region of the ADAM33 gene

Can the tag location of adam33 be customized according to my requirements?

Yes, we can customize the protein according to your requirements.

Will customization requirements affect eligibility for the promotion?

Our current promotion is available for all products and services.

Does the purchased ADAM33 protein have endotoxin?

Depending on your needs, we offer endotoxin removal services

Can the purity of the protein be adjusted according to my requirements?

Yes, we can customize proteins with different purity levels according to your specific needs.

Where is ADAM33 expressed in large quantities?

ADAM33 is abundantly expressed in mesenchymal cells and smooth muscle cells

Which one of His tag or GST tag has less effect on protein activity?

His tag has a smaller molecular weight and has a smaller effect on protein activity.