Recombinant Human ADAMTS13 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ADAMTS13-1432H
• Product Overview: ADAMTS13 MS Standard C13 and N15-labeled recombinant protein (NP_620594) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 149.9 kDa
• AA Sequence: MHQRHPRARCPPLCVAGILACGFLL GCWGPSHFQQSCLQALEPQAVSSYL SPGAPLKGRPPSPGFQRQRQRQRRA AGGILHLELLVAVGPDVFQAHQEDT ERYVLTNLNIGAELLRDPSLGAQFR VHLVKMVILTEPEGAPNITANLTSS LLSVCGWSQTINPEDDTDPGHADLV LYITRFDLELPDGNRQVRGVTQLGG ACSPTWSCLITEDTGFDLGVTIAHE IGHSFGLEHDGAPGSGCGPSGHVMA SDGAAPRAGLAWSPCSRRQLLSLLS AGRARCVWDPPRPQPGSAGHPPDAQ PGLYYSANEQCRVAFGPKAVACTFA REHLDMCQALSCHTDPLDQSSCSRL LVPLLDGTECGVEKWCSKGRCRSLV ELTPIAAVHGRWSSWGPRSPCSRSC GGGVVTRRRQCNNPRPAFGGRACVG ADLQAEMCNTQACEKTQLEFMSQQC ARTDGQPLRSSPGGASFYHWGAAVP HSQGDALCRHMCRAIGESFIMKRGD SFLDGTRCMPSGPREDGTLSLCVSG SCRTFGCDGRMDSQQVWDRCQVCGG DNSTCSPRKGSFTAGRAREYVTFLT VTPNLTSVYIANHRPLFTHLAVRIG GRYVVAGKMSISPNTTYPSLLEDGR VEYRVALTEDRLPRLEEIRIWGPLQ EDADIQVYRRYGEEYGNLTRPDITF TYFQPKPRQAWVWAAVRGPCSVSCG AGLRWVNYSCLDQARKELVETVQCQ GSQQPPAWPEACVLEPCPPYWAVGD FGPCSASCGGGLRERPVRCVEAQGS LLKTLPPARCRAGAQQPAVALETCN PQPCPARWEVSEPSSCTSAGGAGLA LENETCVPGADGLEAPVTEGPGSVD EKLPAPEPCVGMSCPPGWGHLDATS AGEKAPSPWGSIRTGAQAAHVWTPA AGSCSVSCGRGLMELRFLCMDSALR VPVQEELCGLASKPGSRREVCQAVP CPARWQYKLAACSVSCGRGVVRRIL YCARAHGEDDGEEILLDTQCQGLPR PEPQEACSLEPCPPRWKVMSLGPCS ASCGLGTARRSVACVQLDQGQDVEV DEAACAALVRPEASVPCLIADCTYR WHVGTWMECSVSCGDGIQRRRDTCL GPQAQAPVPADFCQHLPKPVTVRGC WAGPCVGQGTPSLVPHEEAAAPGRT TATPAGASLEWSQARGLLFSPAPQP RRLLPGPQENSVQSSACGRQHLEPT GTIDMRGPGQADCAVAIGRPLGEVV TLRVLESSLNCSAGDMLLLWGRLTW RKMCRKLLDMTFSSKTNTLVVRQRC GRPGGGVLLRYGSQLAPETFYRECD MQLFGPWGEIVSPSLSPATSNAGGC RLFINVAPHARIAIHALATNMGAGT EGANASYILIRDTHSLRTTAFHGQQ VLYWESESSQAEMEFSEGFLKAQAS LRGQYWTLQSWVPEMQDPQSWKGKE GTTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ADAMTS13 ADAM metallopeptidase with thrombospondin type 1 motif 13 [ Homo sapiens (human) ]
• Official Symbol: ADAMTS13
• Synonyms: ADAMTS13; ADAM metallopeptidase with thrombospondin type 1 motif, 13; a disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13, C9orf8; A disintegrin and metalloproteinase with thrombospondin motifs 13; DKFZp434C2322; FLJ42993; MGC118899; MGC118900; TTP; vWF CP; VWFCP; ADAM-TS 13; vWF-cleaving protease; von Willebrand factor-cleaving protease; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13; 13; C9orf8; vWF-CP; ADAM-TS; ADAM-TS13; ADAMTS-13
• Gene ID: 11093
• mRNA Refseq: NM_139025
• Protein Refseq: NP_620594
• MIM: 604134
• UniProt ID: Q76LX8

Reviews

07/21/2021

The ADAM6TS13 proteins purchased from your company is suitable for ELISA.

07/10/2021

Customer service was patient and careful, solved all kinds of problems for me.

06/12/2021

The technician answered my questions in detail and helped me to get the desired results.

Q&As

Will all ADAMTS13 participate in this event?

Yes, all ADAMTS13 are eligible for this promotion, including proteins that require custom production.

Are there other expression systems available for ADAMTS13?

We have multiple expression systems, including bacterial expression systems, baculovirus-insect cell expression systems, cell-free in vitro protein expression systems, mammalian expression systems, yeast expression systems, etc.

Where is ADAMTS13 mainly synthesized?

ADAMTS13 is mainly synthesized in the liver.

What are the components of ADAMTS13?

ADAMTS13 consists of signal peptide, leader peptide, metalloproteinase domain, disintegrin-like domain, thrombospondin domain 1, a cysteine-rich domain, a spacer, a TSP motif region and two CUB domains.

Where is ADAMTS13 mainly located?

Using mCherry fluorescent protein labeling of ADAMTS13, it was found that it was mainly localized to the top of the thrombus.

What is the biological function of ADAMTS13?

ADAMTS13 can inhibit the inflammatory response by cleaving vascular hemophilic factors to reduce inflammatory response and thus reduce vascular endothelial function deficits.

What is ADAMTS13 also called?

ADAMTS13 cleaves the active supervascular hemophilia factor (vWF) and is therefore also known as vWF cleavage protease.

How should I choose among different ADAMTS13?

Our experts will recommend suitable products for you based on your research needs.

What diseases is ADAMTS13 involved in?

ADAMTS13 is involved in the study of diseases such as chronic kidney disease and diabetes.