Recombinant Human ADAMTS3 293 Cell Lysate

• Cat.No.: ADAMTS3-9030HCL

Specification

• Description: Antigen standard for ADAM metallopeptidase with thrombospondin type 1 motif, 3 (ADAMTS3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADAMTS3 ADAM metallopeptidase with thrombospondin type 1 motif, 3 [ Homo sapiens ]
• Official Symbol: ADAMTS3
• Synonyms: ADAMTS3; ADAM metallopeptidase with thrombospondin type 1 motif, 3; a disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 3; A disintegrin and metalloproteinase with thrombospondin motifs 3; ADAMTS 4; KIAA0366; ADAM-TS3; ADAMTS-3; PC II-NP; ADAM-TS 3; zinc metalloendopeptidase; procollagen II N-proteinase; procollagen II amino propeptide-processing enzyme; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 3; ADAMTS-4
• Gene ID: 9508
• mRNA Refseq: NM_014243
• Protein Refseq: NP_055058
• MIM: 605011
• UniProt ID: O15072
• Chromosome Location: 4q21
• Function: heparin binding; metal ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding

Reviews

12/25/2021

Friendly customer service and detailed answers to all the questions I asked!

05/15/2020

Your company offers a one-stop shop and the process from purchase to receipt of the protein was very smooth!

07/20/2019

The purchased ADAMTS3 protein is suitable for WB experiments and I am satisfied with the results.

Q&As

Which proteins have the highest homology to ADAMTS3?

ADAMTS2, ADAMTS3 and ADAMTS14 had high homology.

What are the effects of a lack of ADAMTS3?

ADAMTS3 is required for lymphangiogenesis and its defective function leads to abnormal lymphatic vessel development.

Where is ADAMTS3 mainly expressed?

ADAMTS3 is widely expressed in connective tissues such as bone and tendon.

What is the function of ADAMTS3?

ADAMTS3 is a procollagen N-propeptidase that is essential for the maturation of triple-helical collagen fibrils.