Recombinant Human ADD2 Protein, Myc/DDK-tagged, C13 and N15-labeled
Cat.No. : | ADD2-6323H |
Product Overview : | ADD2 MS Standard C13 and N15-labeled recombinant protein (NP_059522) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells. |
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Description : | Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. |
Source : | HEK293 |
Species : | Human |
Tag : | Myc/DDK |
Molecular Mass : | 72.5 kDa |
AA Sequence : | MSEETVPEAASPPPPQGQPYFDRFS EDDPEYMRLRNRAADLRQDFNLMEQ KKRVTMILQSPSFREELEGLIQEQM KKGNNSSNIWALRQIADFMASTSHA VFPTSSMNVSMMTPINDLHTADSLN LAKGERLMRCKISSVYRLLDLYGWA QLSDTYVTLRVSKEQDHFLISPKGV SCSEVTASSLIKVNILGEVVEKGSS CFPVDTTGFCLHSAIYAARPDVRCI IHLHTPATAAVSAMKWGLLPVSHNA LLVGDMAYYDFNGEMEQEADRINLQ KCLGPTCKILVLRNHGVVALGDTVE EAFYKIFHLQAACEIQVSALSSAGG VENLILLEQEKHRPHEVGSVQWAGS TFGPMQKSRLGEHEFEALMRMLDNL GYRTGYTYRHPFVQEKTKHKSEVEI PATVTAFVFEEDGAPVPALRQHAQK QQKEKTRWLNTPNTYLRVNVADEVQ RSMGSPRPKTTWMKADEVEKSSSGM PIRIENPNQFVPLYTDPQEVLEMRN KIREQNRQDVKSAGPQSQLLASVIA EKSRSPSTESQLMSKGDEDTKDDSE ETVPNPFSQLTDQELEEYKKEVERK KLELDETGQEREPGSGPAVCEFFSV ALHIWSNILERKKLPQKSLAHLQSL HLLLQCRAQRRRQRQRALTRTRPLE QKLISEEDLAANDILDYKDDDDKV |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : | Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : | 50 μg/mL as determined by BCA |
Storage Buffer : | 100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
Gene Name : | ADD2 adducin 2 [ Homo sapiens (human) ] |
Official Symbol : | ADD2 |
Synonyms : | ADD2; adducin 2 (beta); beta-adducin; ADDB; erythrocyte adducin subunit beta; |
Gene ID : | 119 |
mRNA Refseq : | NM_017488 |
Protein Refseq : | NP_059522 |
MIM : | 102681 |
UniProt ID : | P35612 |
Products Types
◆ Recombinant Protein | ||
Add2-540M | Recombinant Mouse Add2 Protein, MYC/DDK-tagged | +Inquiry |
ADD2-71R | Recombinant Rhesus Macaque ADD2 Protein, His (Fc)-Avi-tagged | +Inquiry |
ADD2-340M | Recombinant Mouse ADD2 Protein, His (Fc)-Avi-tagged | +Inquiry |
ADD2-337H | Recombinant Human ADD2 Protein, GST-tagged | +Inquiry |
ADD2-301607H | Recombinant Human ADD2 protein, GST-tagged | +Inquiry |
◆ Lysates | ||
ADD2-9015HCL | Recombinant Human ADD2 293 Cell Lysate | +Inquiry |
ADD2-9016HCL | Recombinant Human ADD2 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionThere may exist small molecules or drugs that selectively modulate the activity or stability of ADD2.
ADD2 interacts with specific proteins or binding partners, and these interactions can be context-dependent.
The expression profile of ADD2 varies across different tissues and cell types.
ADD2 regulates downstream signaling pathways or target molecules that contribute to its cellular functions.
Specific antibodies or probes can be developed to visualize the localization or dynamics of ADD2 in live cells or tissues.
Post-translational modifications, such as phosphorylation or ubiquitination, occur on ADD2 and can influence its function.
Functional domains or motifs within ADD2 can be identified and characterized.
The expression of ADD2 can be regulated during development or in response to specific stimuli.
Modulating the expression or activity of ADD2 can impact cellular processes like cytoskeletal organization, cell motility, or membrane dynamics.
ADD2 exhibits subcellular localization, and its localization may undergo dynamic changes or translocation.
Customer Reviews (2)
Write a reviewHighly sensitive in bioactivity assays, accurately detecting the activity and concentration of bioactive factors.
Provides accurate and reliable quantification of protein biomarkers in clinical samples.
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