Recombinant Human ADGRE2 protein, His & T7-tagged

• Cat.No.: ADGRE2-1748H
• Product Overview: Recombinant Human ADGRE2 aa. (Asn347~Ser509 (Accession # Q9UHX3)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 21.5kDa.
• Protein length: Asn347~Ser509 (Accession # Q9UHX3)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.4.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: ADGRE2 adhesion G protein-coupled receptor E2 [ Homo sapiens (human) ]
• Official Symbol: ADGRE2
• Synonyms: Car12; carbonic anhydrase 12; carbonic anyhydrase 12; membrane-bound carbonic anhydrase 12
• Gene ID: 30817
• mRNA Refseq: NM_001271052.1
• Protein Refseq: NP_001257981.1
• UniProt ID: Q9UHX3

Reviews

09/24/2021

Demonstrates excellent stability and consistency in long-term storage.

03/15/2021

Provides accurate and reproducible quantification of the target protein in mass spectrometry analysis.

Q&As

Does ADGRE2 play a role in immune cell activation, migration, or adhesion, and what are the underlying mechanisms?

ADGRE2 plays a role in immune cell activation, migration, and adhesion through mechanisms involving [specific cellular processes or adhesion molecules].

What are the functional consequences of ADGRE2 activation or inhibition on immune cell function and immune responses?

Activation or inhibition of ADGRE2 has functional consequences on immune cell function and immune responses, impacting [specific immune cell activities or immune response outcomes].

What are the downstream signaling pathways activated by ADGRE2 and how do they contribute to cellular processes?

ADGRE2 activates specific downstream signaling pathways, including [specific signaling cascades], which contribute to various cellular processes, such as [specific immune cell activation or migration].

Are there any known ligands or interacting partners that bind to the ADGRE2 protein and modulate its function?

ADGRE2 interacts with specific ligands or interacting partners, such as [specific molecules or cell surface receptors], leading to the modulation of its function.

Can the expression or activity of ADGRE2 be modulated by pharmacological agents or genetic manipulation?

The expression or activity of ADGRE2 can be modulated by pharmacological agents that target [specific receptors or signaling pathways], or through genetic manipulation using [specific genetic tools or techniques].

What is the impact of ADGRE2 deficiency or knockout on immune cell phenotypes or immune responses?

Deficiency or knockout of ADGRE2 in immune cells leads to observable changes in immune cell phenotypes or altered immune responses, characterized by [specific immune cell dysfunctions or immune response impairments].

Does ADGRE2 play a role in specific diseases or pathological conditions, and if so, what are the associated mechanisms?

ADGRE2 is implicated in specific diseases or pathological conditions, such as [specific autoimmune diseases or inflammatory disorders], where it contributes to [specific disease mechanisms or immune dysregulation].

What is the subcellular localization and distribution pattern of ADGRE2 within immune cells or relevant cell types?

ADGRE2 exhibits a [specific subcellular localization pattern], with localization primarily observed in [specific cellular compartments, such as plasma membrane or endosomes].

How is the expression of ADGRE2 regulated under normal physiological conditions and in response to specific stimuli?

Under normal physiological conditions, the expression of ADGRE2 is tightly regulated and can be modulated in response to specific stimuli, such as [specific cytokines or inflammatory signals].

What is the tissue-specific expression pattern of the ADGRE2 protein across different organs and cell types?

The ADGRE2 protein exhibits a specific expression pattern across various tissues and cell types, with higher levels observed in [specific tissues or cell populations].