Recombinant Human ADH5 Protein, His-tagged
Cat.No. : | ADH5-26H |
Product Overview : | Recombinant Human ADH5 Protein is produced by E. coli expression system. This protein is fused with a His tag. |
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Description : | ADH5 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. Recombinant human ADH5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | Liquid. 20mM Tris-HCl buffer (pH7.5) containing 20% glycerol 0.1M Nacl, 1mM DTT. |
Molecular Mass : | 42.3 kDa |
Protein length : | 1-374 aa |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MGSHMANEVI KCKAAVAWEA GKPLSIEEIE VAPPKAHEVR IKIIATAVCH TDAYTLSGAD PEGCFPVILG HEGAGIVESV GEGVTKLKAG DTVIPLYIPQ CGECKFCLNP KTNLCQKIRV TQGKGLMPDG TSRFTCKGKT ILHYMGTSTF SEYTVVADIS VAKIDPLAPL DKVCLLGCGI STGYGAAVNT AKLEPGSVCA VFGLGGVGLA VIMGCKVAGA SRIIGVDINK DKFARAKEFG ATECINPQDF SKPIQEVLIE MTDGGVDYSF ECIGNVKVMR AALEACHKGW GVSVVVGVAA SGEEIATRPF QLVTGRTWKG TAFGGWKSVE SVPKLVSEYM SKKIKVDEFV THNLSFDEIN KAFELMHSGK SIRTVVKI |
Purity : | > 90% by SDS - PAGE |
Notes : | For research use only. This product is not intended or approved for human, diagnostics or veterinary use. |
Storage : | Can be stored at +2 centigrade to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : | 1.0 mg/ml (determined by Bradford assay) |
Gene Name : | ADH5 alcohol dehydrogenase 5 (class III), chi polypeptide [ Homo sapiens ] |
Official Symbol : | ADH5 |
Synonyms : | ADH5; ADH 3; ADHX; formaldehyde dehydrogenase; FDH; ADH-3; FALDH; GSNOR; GSH-FDH; |
Gene ID : | 128 |
mRNA Refseq : | NM_000671 |
Protein Refseq : | NP_000662 |
MIM : | 103710 |
UniProt ID : | P11766 |
Products Types
◆ Recombinant Protein | ||
ADH5-0257H | Recombinant Human ADH5 Protein (M1-I374), GST tagged | +Inquiry |
ADH5-1370H | Recombinant Human ADH5 Protein, MYC/DDK-tagged | +Inquiry |
ADH5-180R | Recombinant Rat ADH5 Protein, His (Fc)-Avi-tagged | +Inquiry |
ADH5-344M | Recombinant Mouse ADH5 Protein, His (Fc)-Avi-tagged | +Inquiry |
ADH5-1317H | Recombinant Human ADH5 Protein (2-374 aa), His-tagged | +Inquiry |
◆ Lysates | ||
ADH5-30HCL | Recombinant Human ADH5 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionADH5 exhibits a tissue-specific expression pattern, and its expression is regulated by various factors such as transcriptional control and signaling pathways.
ADH5 can efficiently metabolize different alcohol substrates, and its substrate specificity may differ from other ADH enzymes.
ADH5 expression and activity can be influenced by chronic alcohol exposure or withdrawal, and studying the underlying molecular mechanisms can shed light on alcohol-related effects.
The activity or function of ADH5 can potentially be modulated by pharmacological agents or environmental factors, presenting potential therapeutic implications.
Interacting proteins or cofactors can modulate the activity or function of ADH5, and investigating the molecular mechanisms involved can enhance our understanding of its regulation.
The enzymatic kinetics and catalytic properties of ADH5 contribute to its overall activity and efficiency in alcohol metabolism.
ADH5 may have implications in the metabolism of other endogenous compounds or xenobiotics, suggesting its involvement in broader physiological functions.
Genetic variations or polymorphisms in the ADH5 gene may impact alcohol metabolism and susceptibility to alcohol-related disorders, emphasizing the role of genetic factors in its function.
The structural features of ADH5 play a crucial role in its catalytic activity and binding affinity for alcohol, and understanding these features provides insights into its function.
Exploring the consequences of ADH5 deficiency or overexpression provides insights into its role in alcohol metabolism and its impact on physiological processes.
Customer Reviews (2)
Write a reviewHighly effective in stabilizing and enhancing the activity of enzymes in enzyme engineering.
Demonstrates excellent resistance to aggregation and aggregation-prone conditions.
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