Recombinant Human ADSL 293 Cell Lysate

• Cat.No.: ADSL-8995HCL

Specification

• Description: Antigen standard for adenylosuccinate lyase (ADSL), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADSL adenylosuccinate lyase [ Homo sapiens ]
• Official Symbol: ADSL
• Synonyms: ADSL; adenylosuccinate lyase; adenylosuccinase; ASL; AMPS; ASASE
• Gene ID: 158
• mRNA Refseq: NM_000026
• Protein Refseq: NP_000017
• MIM: 608222
• UniProt ID: P30566
• Chromosome Location: 22q13.1
• Pathway: Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Inosine monophosphate biosynthesis, PRPP + glutamine =>IMP, organism-specific biosystem; Inosine monophosphate biosynthesis, PRPP + glutamine => IMP, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate AMP-lyase (fumarate-forming) activity; N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity; NOT N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity; N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity; lyase activity

Reviews

07/31/2021

Exploring protein-protein interactions involved in apoptosis signaling.

03/27/2018

Characterizing protein-protein interactions involved in cancer progression.

01/17/2017

Determining protein-protein interaction interfaces using X-ray crystallography.

Q&As

Are there any known genetic variations or mutations in the Adsl gene that affect protein function or stability?

Genetic variations or mutations in the Adsl gene can affect the stability or function of Adsl protein, leading to metabolic disorders like Adenylosuccinate Lyase Deficiency.

Can Adsl protein interact with other proteins or form protein complexes, and if so, what are their functional implications?

Adsl protein can interact with other proteins and form complexes, including the formation of multiprotein complexes involved in purine metabolism.

What are the structural features of Adsl protein that contribute to its enzymatic activity and substrate specificity?

The enzymatic activity and substrate specificity of Adsl protein are attributed to its unique structural features, including conserved domains and active sites.

What experimental techniques have been used to characterize the enzymatic activity of Adsl protein?

Experimental techniques such as enzymatic assays and substrate analysis have been used to characterize the enzymatic activity of Adsl protein.

Are there any pharmacological agents or small molecules that can modulate the activity or expression of Adsl protein, and could they have therapeutic potential for related conditions?

Currently, there are no specific pharmacological agents targeting Adsl protein. However, understanding its regulation and functional implications may lead to the development of potential therapeutic approaches for related conditions.

What are the specific metabolic pathways or cellular processes in which Adsl protein is involved?

Adsl protein is primarily involved in the de novo synthesis of purine nucleotides and participates in the purine salvage pathway.

Can the cellular localization of Adsl protein change in response to specific signals or cellular conditions?

The cellular localization of Adsl protein is primarily in the cytoplasm, but it can also be found in the nucleus under specific conditions.

How does dysregulation or dysfunction of Adsl protein impact cellular metabolism and contribute to human diseases or disorders?

Dysregulation or dysfunction of Adsl protein can disrupt purine metabolism, leading to metabolic disorders such as Adenylosuccinate Lyase Deficiency, which affects neurological and developmental processes.

How is the expression of Adsl protein regulated at the transcriptional and post-transcriptional levels?

The expression of Adsl protein is regulated at both the transcriptional and post-transcriptional levels by various factors, such as transcription factors and microRNAs.

Are there any known cellular factors or cofactors that modulate the activity of Adsl protein?

Adsl protein activity can be modulated by cellular factors and cofactors, including magnesium ions and ATP.