Recombinant Human ADSS, GST-tagged

• Cat.No.: ADSS-9450H
• Product Overview: Recombinant Human ADSS protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Description: This gene encodes the enzyme adenylosuccinate synthetase which catalyzes the first committed step in the conversion of inosine monophosphate to adenosine monophosphate. A pseudogene of this gene is found on chromosome 17.
• Source: E.coli
• Species: Human
• Tag: GST
• Protein length: 100-456a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: ADSS adenylosuccinate synthase [ Homo sapiens ]
• Official Symbol: ADSS
• Synonyms: adenylosuccinate synthase; L-type adenylosuccinate synthetase; Adenylosuccinate synthetase, acidic isozyme; EC 6.3.4.4; Adenylosuccinate synthetase, liver isozyme; ADEH; AdSS 2; adenylosuccinate synthetase (Ade(-)H-complementing); AMPSase 2; adenylosuccinate synthetase isozyme 2; IMP--aspartate ligase 2; ADSS2
• Gene ID: 159
• mRNA Refseq: NM_001126.3
• Protein Refseq: NP_001117.2
• MIM: 103060
• UniProt ID: P30520
• Chromosome Location: 1q44
• Pathway: Adenine ribonucleotide biosynthesis, IMP =>ADP,ATP, organism-specific biosystem; Adenine ribonucleotide biosynthesis, IMP =>ADP,ATP, conserved biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Metabolism, organism-specific biosystem
• Function: GTP binding; adenylosuccinate synthase activity; adenylosuccinate synthase activity; magnesium ion binding; phosphate ion binding

Reviews

02/02/2022

Understanding protein-protein interaction networks in mitochondrial function.

12/16/2019

Detecting protein-protein interactions in extracellular matrix assembly.

04/01/2018

Exploring protein-protein interactions involved in stem cell differentiation.

Q&As

How is the expression of Adss protein regulated at the transcriptional and post-transcriptional levels?

The expression of Adss protein is regulated at both the transcriptional and post-transcriptional levels by various factors, such as transcription factors and microRNAs.

What are the specific metabolic pathways or cellular processes in which Adss protein is involved?

Adss protein is primarily involved in the biosynthesis of purine nucleotides and participates in the de novo purine synthesis pathway.

Can Adss protein form protein complexes or interact with other proteins, and if so, what are the functional consequences?

Adss protein can potentially form protein complexes or interact with other proteins, which may have functional implications in cellular processes related to purine metabolism.

Are there any pharmacological agents or small molecules that can modulate the activity or expression of Adss protein, and could they have potential therapeutic applications?

Currently, there are no specific pharmacological agents targeting Adss protein. However, further understanding of its regulation and functional implications may lead to the development of potential therapeutic approaches for related conditions.

What experimental techniques have been used to investigate the enzymatic activity of Adss protein?

Experimental techniques such as enzymatic assays and substrate analysis have been used to study the enzymatic activity of Adss protein.

How does dysregulation or dysfunction of Adss protein impact cellular metabolism and contribute to human diseases or disorders?

Dysregulation or dysfunction of Adss protein can disrupt purine metabolism, potentially contributing to disorders involving abnormalities in nucleotide synthesis.

Are there any genetic variations or mutations in the Adss gene that affect protein function or stability?

Genetic variations or mutations in the Adss gene may impact the function or stability of Adss protein, potentially leading to disorders associated with purine metabolism.

Are there any known cellular factors or cofactors that regulate or interact with Adss protein?

Adss protein activity may be regulated or influenced by cellular factors and cofactors, including ATP and magnesium ions.

Can the subcellular localization of Adss protein change under specific cellular conditions or stimuli?

The subcellular localization of Adss protein is predominantly cytoplasmic, but it may undergo changes under specific cellular conditions or stimuli.

What are the structural features of Adss protein that contribute to its enzymatic activity and substrate specificity?

The structural features of Adss protein, including conserved domains and active sites, contribute to its enzymatic activity and substrate specificity in purine biosynthesis.