Recombinant Mouse Adss Protein, MYC/DDK-tagged

• Cat.No.: Adss-551M
• Product Overview: Purified recombinant protein of full-length mouse adenylosuccinate synthetase, non muscle (Adss), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Ubiquitous expression in CNS E18 (RPKM 16.5), CNS E14 (RPKM 15.7) and 28 other tissues.
• Source: HEK293T
• Species: Mouse
• Tag: MYC/DDK
• Molecular Mass: 50.5 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Adss adenylosuccinate synthetase, non muscle [ Mus musculus (house mouse) ]
• Official Symbol: Adss
• Synonyms: Adss; adenylosuccinate synthetase, non muscle; AS; Adss2; AI314886; adenylosuccinate synthetase isozyme 2; AMPSase 2; IMP--aspartate ligase 2; L-type adenylosuccinate synthetase; adSS 2; adenylosuccinate synthetase 2, non muscle; adenylosuccinate synthetase, acidic isozyme; adenylosuccinate synthetase, liver isozyme; adenylosuccinate synthetase, non-muscle isozyme; EC 6.3.4.4
• Gene ID: 11566
• mRNA Refseq: NM_007422
• Protein Refseq: NP_031448
• UniProt ID: P46664

Reviews

02/02/2022

Understanding protein-protein interaction networks in mitochondrial function.

12/16/2019

Detecting protein-protein interactions in extracellular matrix assembly.

04/01/2018

Exploring protein-protein interactions involved in stem cell differentiation.

Q&As

How is the expression of Adss protein regulated at the transcriptional and post-transcriptional levels?

The expression of Adss protein is regulated at both the transcriptional and post-transcriptional levels by various factors, such as transcription factors and microRNAs.

What are the specific metabolic pathways or cellular processes in which Adss protein is involved?

Adss protein is primarily involved in the biosynthesis of purine nucleotides and participates in the de novo purine synthesis pathway.

Can Adss protein form protein complexes or interact with other proteins, and if so, what are the functional consequences?

Adss protein can potentially form protein complexes or interact with other proteins, which may have functional implications in cellular processes related to purine metabolism.

Are there any pharmacological agents or small molecules that can modulate the activity or expression of Adss protein, and could they have potential therapeutic applications?

Currently, there are no specific pharmacological agents targeting Adss protein. However, further understanding of its regulation and functional implications may lead to the development of potential therapeutic approaches for related conditions.

What experimental techniques have been used to investigate the enzymatic activity of Adss protein?

Experimental techniques such as enzymatic assays and substrate analysis have been used to study the enzymatic activity of Adss protein.

How does dysregulation or dysfunction of Adss protein impact cellular metabolism and contribute to human diseases or disorders?

Dysregulation or dysfunction of Adss protein can disrupt purine metabolism, potentially contributing to disorders involving abnormalities in nucleotide synthesis.

Are there any genetic variations or mutations in the Adss gene that affect protein function or stability?

Genetic variations or mutations in the Adss gene may impact the function or stability of Adss protein, potentially leading to disorders associated with purine metabolism.

Are there any known cellular factors or cofactors that regulate or interact with Adss protein?

Adss protein activity may be regulated or influenced by cellular factors and cofactors, including ATP and magnesium ions.

Can the subcellular localization of Adss protein change under specific cellular conditions or stimuli?

The subcellular localization of Adss protein is predominantly cytoplasmic, but it may undergo changes under specific cellular conditions or stimuli.

What are the structural features of Adss protein that contribute to its enzymatic activity and substrate specificity?

The structural features of Adss protein, including conserved domains and active sites, contribute to its enzymatic activity and substrate specificity in purine biosynthesis.