Recombinant Human AFF4 293 Cell Lysate

• Cat.No.: AFF4-8988HCL

Specification

• Description: Antigen standard for AF4/FMR2 family, member 4 (AFF4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AFF4 AF4/FMR2 family, member 4 [ Homo sapiens ]
• Official Symbol: AFF4
• Synonyms: AFF4; AF4/FMR2 family, member 4; AF4/FMR2 family member 4; AF5Q31; ALL1 fused gene from 5q31; MCEF; ALL1-fused gene from chromosome 5q31 protein; major CDK9 elongation factor-associated protein; MGC75036
• Gene ID: 27125
• mRNA Refseq: NM_014423
• Protein Refseq: NP_055238
• MIM: 604417
• UniProt ID: Q9UHB7
• Chromosome Location: 5q31
• Function: protein binding; sequence-specific DNA binding transcription factor activity

Reviews

10/12/2021

Investigating protein-protein interactions in cellular metabolism for energy balance.

03/08/2020

Studying protein-protein interactions in cell migration for tissue morphogenesis.

07/24/2017

Mapping protein-protein interactions in receptor trafficking for cellular signaling.

Q&As

What is the subcellular localization of AFF4 protein, and how can it be experimentally determined?

The subcellular localization of AFF4 protein can be experimentally determined using techniques such as immunofluorescence microscopy or subcellular fractionation.

How is the expression of AFF4 protein regulated, and what factors or signaling pathways control its expression?

The expression of AFF4 protein is regulated by specific factors and signaling pathways.

What experimental techniques or assays have been used to study the functional significance of AFF4 protein, such as chromatin immunoprecipitation, RNA-seq, or gene knockdown approaches?

Various experimental techniques or assays, such as chromatin immunoprecipitation, RNA-seq, or gene knockdown approaches, have been used to study the functional significance of AFF4 protein.

Does AFF4 protein interact with specific molecules or participate in protein complexes, and what are the functional implications of these interactions?

AFF4 protein may interact with specific molecules or participate in protein complexes, which have functional implications in cellular processes.

Are there any known post-translational modifications or regulatory mechanisms that modulate the activity or stability of AFF4 protein?

Post-translational modifications and regulatory mechanisms may modulate the activity or stability of AFF4 protein.

Are there any genetic variations or mutations in the gene encoding AFF4 protein that impact its expression or function, and what are the implications for cellular processes or disease susceptibility?

Genetic variations or mutations in the gene encoding AFF4 protein may impact its expression or function, influencing cellular processes or disease susceptibility.

How does dysregulation or dysfunction of AFF4 protein affect transcriptional regulation, gene expression networks, or disease development?

Dysregulation or dysfunction of AFF4 protein can affect transcriptional regulation, perturb gene expression networks, and contribute to disease development.

How does AFF4 protein contribute to specific cellular processes such as development, differentiation, or disease progression, and what are the underlying molecular mechanisms?

AFF4 protein contributes to specific cellular processes such as development, differentiation, or disease progression, and the underlying molecular mechanisms are currently being investigated.

Can AFF4 protein be targeted or manipulated to modulate gene expression or disease phenotypes, and what are the potential implications for therapeutic interventions?

AFF4 protein holds potential as a target for modulating gene expression or disease phenotypes, and further research is needed to explore its implications for therapeutic interventions.

What is the role of AFF4 protein in transcriptional elongation or chromatin remodeling, and how can its function be investigated experimentally?

AFF4 protein plays a role in transcriptional elongation or chromatin remodeling, and its function can be investigated using techniques like chromatin immunoprecipitation or gene expression analysis.