Recombinant Human AGMAT, His-tagged

• Cat.No.: AGMAT-3241H
• Product Overview: Agmatine Ureohydrolase (AGMAT)

Specification

• Description: About the Marker: Effective Size Range: 10kDa to 70kDa.Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.Ready-to-use: No need to heat, dilute or add reducing agents before use.
• Source: E. Coli
• Species: Human
• Tag: His
• Form: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Molecular Mass: 17.1kDa
• Protein length: 352
• Endotoxin: <1.0eu per 1ug (determined by the lal</1.0eu
• Purity: >95%
• Applications: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Notes: Small volumes of AGMAT recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: AGMAT agmatine ureohydrolase (agmatinase) [ Homo sapiens ]
• Official Symbol: AGMAT
• Synonyms: AGMAT; agmatine ureohydrolase (agmatinase); agmatinase, mitochondrial; FLJ23384; AUH
• Gene ID: 79814
• mRNA Refseq: NM_024758
• Protein Refseq: NP_079034
• UniProt ID: Q9BSE5
• Chromosome Location: 1p36.13
• Pathway: Agmatine biosynthesis, organism-specific biosystem; Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosyste
• Function: agmatinase activity; hydrolase activity; metal ion binding

Reviews

08/27/2022

 The protein product’s ability to induce apoptosis provided valuable insights into cell mechanisms.

04/17/2022

 Precise results from immunoprecipitation assays were attributed to the protein product’s purity.

02/26/2022

 Well-defined gel electrophoresis bands were indicative of the protein product’s pure form.

10/08/2018

 Protein interactions were effectively modulated by the protein product, broadening our understanding.

06/25/2017

The insights gained from the protein product justified its cost, offering value for money.

Q&As

How does AGMAT's deficiency impact polyamine levels?

AGMAT deficiency can lead to elevated agmatine levels and disrupted polyamine balance.

Can AGMAT's activity influence wound healing?

Yes, AGMAT's involvement in polyamine metabolism could impact wound healing processes.

What's the connection between AGMAT and agmatine?

AGMAT converts agmatine to putrescine, a crucial step in agmatine metabolism.

How does AGMAT contribute to cellular function?

AGMAT's activity helps regulate polyamine levels, impacting various cellular processes.

Can AGMAT impact brain health?

Yes, AGMAT's role in polyamine metabolism could have implications for brain function.

How is AGMAT deficiency inherited?

AGMAT deficiency is inherited in an autosomal recessive manner.

How is AGMAT's function conserved across species?

Research explores whether AGMAT's role is conserved in different organisms.

Can AGMAT's function be influenced by cellular signaling pathways?

Yes, cellular signaling pathways might regulate AGMAT's activity.

Can AGMAT's activity be modulated for therapeutic purposes?

Research investigates modulating AGMAT activity for potential therapeutic applications.

How does AGMAT impact cellular growth?

AGMAT's role in polyamine metabolism can influence cellular growth and proliferation.

What is the AGMAT protein?

AGMAT stands for "Agmatinase," an enzyme involved in the polyamine metabolism pathway.

How is AGMAT's enzymatic activity studied?

Research investigates AGMAT's enzymatic function and its implications.

Can AGMAT mutations lead to diseases?

Mutations in the AGMAT gene can cause AGMAT deficiency, impacting polyamine metabolism.

What's the role of AGMAT in polyamine metabolism?

AGMAT catalyzes the conversion of agmatine to putrescine, a step in polyamine breakdown.

Can AGMAT be targeted for therapeutic interventions?

Research explores AGMAT as a potential target in diseases related to polyamine metabolism.

Can AGMAT's deficiency affect cellular stress responses?

AGMAT deficiency might impact cellular responses to stress due to altered polyamine metabolism.

Where is AGMAT located in the cell?

AGMAT is located in the cytoplasm of cells.

How does AGMAT's activity relate to putrescine levels?

AGMAT's enzymatic activity directly affects putrescine levels in cells.

Is AGMAT involved in cancer research?

AGMAT's involvement in polyamine metabolism could be relevant in cancer studies.

How is AGMAT's deficiency diagnosed?

Diagnosis involves measuring AGMAT activity in blood or genetic testing.