Recombinant Human AGPAT2 293 Cell Lysate

• Cat.No.: AGPAT2-8977HCL

Specification

• Description: Antigen standard for 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta) (AGPAT2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AGPAT2 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta) [ Homo sapiens ]
• Official Symbol: AGPAT2
• Synonyms: AGPAT2; 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta); Berardinelli Seip congenital lipodystrophy , BSCL; 1-acyl-sn-glycerol-3-phosphate acyltransferase beta; LPAAT beta; 1-AGPAT 2; 1-AGP acyltransferase 2; lysophosphatidic acid acyltransferase beta; lysophosphatidic acid acyltransferase-beta; 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase-beta); BSCL; BSCL1; LPAAB; 1-AGPAT2; LPAAT-beta
• Gene ID: 10555
• mRNA Refseq: NM_001012727
• Protein Refseq: NP_001012745
• MIM: 603100
• UniProt ID: O15120
• Chromosome Location: 9q34.3
• Pathway: Adipogenesis, organism-specific biosystem; CDP-diacylglycerol biosynthesis I, organism-specific biosystem; CDP-diacylglycerol biosynthesis I, conserved biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Glycerolipid metabolism, organism-specific biosystem
• Function: 1-acylglycerol-3-phosphate O-acyltransferase activity; 1-acylglycerol-3-phosphate O-acyltransferase activity; transferase activity, transferring acyl groups

Reviews

05/01/2022

 The product’s quality was evident in the absence of background interference in our assays.

10/23/2021

 The product’s cost-effectiveness and clear instructions were valuable in our experiments.

06/18/2021

 The benefits reaped from the product justified its price in terms of scientific progress.

04/09/2020

Consistent outcomes bolstered the reliability of our conclusions, thanks to the product’s performance.

06/26/2016

 Clear, distinct gel bands in electrophoresis highlighted the product’s pure form.

Q&As

How is AGPAT2’s involvement in lipid droplet formation studied

Research investigates AGPAT2’s impact on lipid droplet dynamics and accumulation.

Can AGPAT2’s activity influence brain lipid composition

Yes, AGPAT2’s function can impact brain lipid composition and neurological functions.

How does AGPAT2’s role intersect with phospholipid remodeling

AGPAT2 contributes to phospholipid biosynthesis and remodeling pathways.

How does AGPAT2’s function relate to lipodystrophy

Mutations in AGPAT2 can cause lipodystrophy, leading to abnormal fat tissue distribution.

How does AGPAT2 contribute to phospholipid synthesis

AGPAT2 adds fatty acids to glycerol-3-phosphate, forming lysophosphatidic acid (LPA).

Can AGPAT2’s dysfunction lead to metabolic disorders

Yes, AGPAT2 mutations can result in metabolic disorders and lipodystrophic phenotypes.

Does AGPAT2 contribute to the regulation of inflammation

Yes, AGPAT2’s activity might impact inflammation through lipid metabolism pathways.

How does AGPAT2’s role relate to liver steatosis

AGPAT2’s function can be involved in hepatic lipid accumulation and fatty liver disease.

How is AGPAT2’s function studied in metabolic diseases

Research explores AGPAT2’s role in metabolic disorders like obesity, diabetes, and lipodystrophy.

What’s the role of AGPAT2 in lipid metabolism

AGPAT2 catalyzes the acylation of glycerol-3-phosphate, a crucial step in phospholipid synthesis.

How is AGPAT2’s involvement in triglyceride synthesis studied

Research explores AGPAT2’s contribution to triglyceride synthesis and energy storage.

How does AGPAT2 contribute to lipid composition of tissues

AGPAT2’s activity influences the lipid composition of various tissues.

How is AGPAT2’s connection to lipogenesis studied

Research explores AGPAT2’s role in de novo lipogenesis and fatty acid synthesis.

Is AGPAT2 essential for normal cellular function

Yes, AGPAT2 is crucial for lipid metabolism and the synthesis of cellular membranes.

Can AGPAT2 be targeted for therapeutic interventions

Yes, AGPAT2 is a potential target for modulating lipid metabolism and related diseases.

Can AGPAT2 play a role in membrane fusion processes

Yes, AGPAT2’s involvement in lipid synthesis can influence membrane fusion and trafficking.

Where is AGPAT2 located in cells

AGPAT2 is primarily located in the endoplasmic reticulum (ER) membrane.

How is AGPAT2’s involvement in glycerophospholipid metabolism studied

Research investigates AGPAT2’s role in lipid biosynthesis pathways.

Can AGPAT2’s activity impact cellular membranes

Yes, AGPAT2’s function is essential for maintaining the composition and integrity of cellular membranes.

Can AGPAT2 influence adipocyte differentiation

Yes, AGPAT2’s activity is linked to adipocyte differentiation and fat tissue development.

How is AGPAT2’s structure characterized

AGPAT2 contains transmembrane domains that allow it to function within membranes.