Recombinant Human AGPAT3 293 Cell Lysate

• Cat.No.: AGPAT3-8976HCL

Specification

• Description: Antigen standard for 1-acylglycerol-3-phosphate O-acyltransferase 3 (AGPAT3), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AGPAT3 1-acylglycerol-3-phosphate O-acyltransferase 3 [ Homo sapiens ]
• Official Symbol: AGPAT3
• Synonyms: AGPAT3; 1-acylglycerol-3-phosphate O-acyltransferase 3; 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma; LPAAT gamma; 1-AGPAT 3; 1-AGP acyltransferase 3; lysophosphatidic acid acyltransferase gamma; lysophosphatidic acid acyltransferase-gamma1; LPAAT-GAMMA1; MGC4604
• Gene ID: 56894
• mRNA Refseq: NM_001037553
• Protein Refseq: NP_001032642
• UniProt ID: Q9NRZ7
• Chromosome Location: 21q22.3
• Pathway: CDP-diacylglycerol biosynthesis I, organism-specific biosystem; CDP-diacylglycerol biosynthesis I, conserved biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Glycerolipid metabolism, organism-specific biosystem; Glycerolipid metabolism, conserved biosystem; Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem
• Function: 1-acylglycerol-3-phosphate O-acyltransferase activity; transferase activity, transferring acyl groups

Reviews

10/14/2022

 The product’s innovative traits allowed us to explore previously unknown cellular processes.

08/30/2021

 Replicating results confirmed the product’s reliability and significance in our research.

08/31/2019

The knowledge gained from the product justified its cost, offering substantial value for investment.

06/24/2018

Feel free to continue expanding your list by adding these reviews to your Excel sheet.

06/20/2016

The product’s consistent outcomes reinforced the reproducibility of our scientific investigations.

Q&As

Does AGPAT3 contribute to phospholipid diversity

Yes, AGPAT3’s involvement helps generate a diverse range of phospholipids for various cellular functions.

Can AGPAT3 be targeted for therapeutic interventions

Yes, AGPAT3 is a potential target for modulating lipid metabolism and related diseases.

Can AGPAT3 impact cellular responses to stress

Yes, AGPAT3’s function can be influenced by cellular stress and changes in metabolic state.

Can AGPAT3’s dysfunction lead to lipid-related diseases

Yes, mutations in AGPAT3 can result in metabolic disorders affecting lipid metabolism and cellular health.

Can AGPAT3’s function be regulated by cellular signals

Yes, AGPAT3’s activity can be regulated by intracellular signals that impact lipid metabolism.

How does AGPAT3’s activity affect cellular homeostasis

AGPAT3’s role in lipid metabolism contributes to maintaining lipid balance and cellular health.

How does AGPAT3’s role affect membrane properties

AGPAT3’s involvement can impact the physical and chemical properties of cellular membranes.

Can AGPAT3 contribute to the regulation of inflammation

Yes, AGPAT3’s activity might impact inflammation through its influence on lipid metabolism.

Is AGPAT3’s involvement conserved across species

Research explores whether AGPAT3’s function is conserved across different organisms.

How is AGPAT3’s role studied in the context of lipidomics

Research explores AGPAT3’s impact on the lipid composition and profiles of cellular membranes.

How does AGPAT3’s activity relate to phospholipid remodeling

AGPAT3 contributes to the synthesis of new phospholipids and the remodeling of existing ones.

Is AGPAT3 involved in cellular membrane formation

Yes, AGPAT3’s function contributes to the synthesis of cellular membranes and lipid bilayers.

How does AGPAT3’s activity relate to lipid signaling

AGPAT3’s role in lipid metabolism can influence the production of lipid signaling molecules.

How does AGPAT3’s role connect to lipotoxicity

AGPAT3’s activity can impact cellular susceptibility to lipotoxicity and lipid-induced damage.

Does AGPAT3 play a role in energy storage

Yes, AGPAT3’s function is linked to energy storage and utilization through lipid metabolism.

Can AGPAT3 contribute to lipid droplet formation

Yes, AGPAT3’s function can influence the formation and dynamics of lipid droplets in cells.

How does AGPAT3’s activity relate to membrane structure

AGPAT3’s role in lipid biosynthesis influences the composition and fluidity of cellular membranes.

Where is AGPAT3 located within cells

AGPAT3 is primarily located in the endoplasmic reticulum (ER) membrane.

Can AGPAT3’s function influence cellular communication

Yes, AGPAT3’s role in lipid metabolism can impact intercellular communication and signaling.

Is AGPAT3’s function linked to cell survival pathways

Yes, AGPAT3’s function can contribute to cell survival through lipid metabolism pathways.

How is AGPAT3’s role in lipid metabolism studied

Research investigates AGPAT3’s function using biochemical assays and cellular experiments.

How is AGPAT3’s impact on lipid bilayer stability studied

Research investigates how AGPAT3’s activity affects the stability and integrity of lipid bilayers.