Recombinant Human AGPAT4 293 Cell Lysate

• Cat.No.: AGPAT4-8975HCL

Specification

• Description: Antigen standard for 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta) (AGPAT4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AGPAT4 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta) [ Homo sapiens ]
• Official Symbol: AGPAT4
• Synonyms: AGPAT4; 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta); 1-acyl-sn-glycerol-3-phosphate acyltransferase delta; dJ473J16.2; LPAAT delta; 1-AGPAT 4; 1-AGP acyltransferase 4; lysophosphatidic acid acyltransferase delta; lysophosphatidic acid acyltransferase-delta (LPAAT-delta); 1-AGPAT4; LPAAT-delta; RP3-473J16.2
• Gene ID: 56895
• mRNA Refseq: NM_020133
• Protein Refseq: NP_064518
• UniProt ID: Q9NRZ5
• Chromosome Location: 6q25.3
• Pathway: CDP-diacylglycerol biosynthesis I, organism-specific biosystem; CDP-diacylglycerol biosynthesis I, conserved biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Glycerolipid metabolism, organism-specific biosystem; Glycerolipid metabolism, conserved biosystem; Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem
• Function: 1-acylglycerol-3-phosphate O-acyltransferase activity; transferase activity, transferring acyl groups

Reviews

10/12/2021

 The comprehensive documentation streamlined the integration of the product into our experimental workflow.

10/07/2018

The knowledge gained from the product justified its cost, offering substantial value for investment.

09/26/2017

 Replicating results emphasized the reliability and relevance of the product’s effects.

01/07/2017

 The product consistently met our expectations, lending credibility to our findings.

09/13/2016

 Comprehensive documentation streamlined our incorporation of the product into our research workflow.

Q&As

How does AGPAT4’s role intersect with lipid storage

AGPAT4’s function can affect cellular lipid storage and energy utilization.

Can AGPAT4 play a role in cellular signaling pathways

Yes, AGPAT4’s activity can impact lipid-mediated signaling processes within cells.

Can AGPAT4 impact lipid bilayer composition

Yes, AGPAT4’s role influences the composition and fluidity of lipid bilayers.

How is AGPAT4’s role linked to lipid metabolism

AGPAT4’s involvement is tied to the regulation of lipid metabolism and homeostasis.

Is AGPAT4’s role associated with organelle integrity

Yes, AGPAT4’s involvement is connected to maintaining the integrity of organelle membranes.

Is AGPAT4’s function crucial for membrane formation

Yes, AGPAT4’s activity is essential for the synthesis and maintenance of cellular membranes.

What is the function of AGPAT4

AGPAT4 is an enzyme involved in the biosynthesis of phospholipids and lipid metabolism.

How does AGPAT4’s role relate to lipid second messengers

AGPAT4’s involvement can impact the production of lipid-derived second messenger molecules.

How is AGPAT4’s activity studied in disease contexts

Research investigates how AGPAT4’s dysfunction contributes to disease mechanisms.

How is AGPAT4’s function studied in lipidomics

Research investigates AGPAT4’s impact on lipid composition and profiles of cellular membranes.

Is AGPAT4’s involvement related to lipid droplet dynamics

Yes, AGPAT4’s function can influence the dynamics and formation of lipid droplets.

How does AGPAT4 contribute to lipid synthesis

AGPAT4 catalyzes the addition of fatty acids to glycerol-3-phosphate, forming LPA.

Can AGPAT4 be linked to lipid-related diseases

Yes, mutations in AGPAT4 can lead to metabolic disorders affecting lipid metabolism.

Can AGPAT4’s function be involved in cellular growth

Yes, AGPAT4’s activity can influence cellular growth and proliferation through lipid metabolism.

How does AGPAT4 contribute to phospholipid remodeling

AGPAT4’s role involves generating diverse phospholipids that contribute to remodeling.

Does AGPAT4 contribute to cellular response to stress

Yes, AGPAT4’s function can be influenced by cellular stress and changes in metabolic state.

Does AGPAT4 play a role in lipid-mediated signaling

Yes, AGPAT4’s activity can contribute to lipid-mediated signaling pathways within cells.

Can AGPAT4’s activity be regulated by cellular signals

Yes, AGPAT4’s function can be modulated by intracellular signals and metabolic cues.

Where is AGPAT4 typically located in cells

AGPAT4 is primarily found in the endoplasmic reticulum (ER) membrane.

How is AGPAT4’s impact on cellular health studied

Research explores how AGPAT4’s function influences cellular health and function.

Can AGPAT4’s activity be targeted for therapeutics

Yes, AGPAT4 is a potential therapeutic target for modulating lipid metabolism and related diseases.

How does AGPAT4 contribute to lipid homeostasis

AGPAT4’s activity helps maintain lipid balance and cellular lipid levels.

How is AGPAT4’s function connected to membrane dynamics

AGPAT4’s role can influence the dynamic behavior and curvature of cellular membranes.

Does AGPAT4’s function vary in different cell types

Yes, AGPAT4’s activity and role can vary based on the specific cellular context.