Recombinant Human AGPAT5 293 Cell Lysate

• Cat.No.: AGPAT5-8974HCL

Specification

• Description: Antigen standard for 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) (AGPAT5) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AGPAT5 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) [ Homo sapiens ]
• Official Symbol: AGPAT5
• Synonyms: AGPAT5; 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon); 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon; FLJ11210; LPAAT e; LPAAT epsilon; 1-AGPAT 5; 1-AGP acyltransferase 5; lysophosphatidic acid acyltransferase epsilon; LPAATE; 1AGPAT5
• Gene ID: 55326
• mRNA Refseq: NM_018361
• Protein Refseq: NP_060831
• UniProt ID: Q9NUQ2
• Chromosome Location: 8p23.1
• Pathway: CDP-diacylglycerol biosynthesis I, organism-specific biosystem; CDP-diacylglycerol biosynthesis I, conserved biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Triacylglyceride Synthesis, organism-specific biosystem; Triglyceride Biosynthesis, organism-specific biosystem
• Function: 1-acylglycerol-3-phosphate O-acyltransferase activity; transferase activity, transferring acyl groups

Reviews

12/25/2021

 The product’s quality was evident in the absence of background interference in our assays.

05/28/2021

The product’s high purity enabled accurate quantification, minimizing data variability.

08/03/2020

 Replicating results confirmed the product’s reliability and significance in our research.

08/19/2018

 Reliable protein-protein interactions were achieved due to the product’s pure composition.

10/05/2017

 The product’s innovative traits allowed us to explore previously unknown cellular processes.

Q&As

How is AGPAT5’s impact on cellular health studied

Research explores how AGPAT5’s function influences overall cellular health and function.

Does AGPAT5 play a role in membrane remodeling

Yes, AGPAT5’s function can influence the remodeling of cellular membranes.

Is AGPAT5’s function connected to cellular signaling

Yes, AGPAT5’s involvement can influence lipid-mediated signaling processes within cells.

Can AGPAT5’s dysfunction lead to lipid-related diseases

Yes, mutations in AGPAT5 can result in metabolic disorders affecting lipid metabolism.

Can AGPAT5 influence cellular membrane composition

Yes, AGPAT5’s activity affects the composition and properties of cellular membranes.

How does AGPAT5 contribute to lipid biosynthesis

AGPAT5 adds fatty acids to glycerol-3-phosphate, forming lysophosphatidic acid (LPA).

How is AGPAT5’s involvement in lipid metabolism studied

Research investigates AGPAT5’s role in lipid metabolism through cellular and biochemical assays.

How does AGPAT5’s activity affect lipid bilayer properties

AGPAT5’s function influences the composition and fluidity of cellular lipid bilayers.

Is AGPAT5 involved in phospholipid remodeling

Yes, AGPAT5’s activity contributes to the generation of diverse phospholipids for cellular functions.

Can AGPAT5’s activity be modulated during stress responses

Yes, AGPAT5’s function can be regulated by cellular stress and metabolic changes.

What is the role of AGPAT5 in cells

AGPAT5 plays a key role in lipid metabolism by catalyzing the synthesis of phospholipids.

How is AGPAT5’s function linked to lipid homeostasis

AGPAT5’s involvement contributes to maintaining lipid balance and cellular health.

How does AGPAT5’s involvement relate to lipid second messengers

AGPAT5’s role can impact the production of lipid-derived second messenger molecules.

What is AGPAT5’s connection to lipid signaling pathways

AGPAT5’s role in lipid metabolism can impact lipid-mediated signaling processes within cells.

Can AGPAT5’s role impact cellular response to stress

Yes, AGPAT5’s function can be influenced by cellular stress and changes in metabolic state.

Where is AGPAT5 localized within cells

AGPAT5 is primarily located in the endoplasmic reticulum (ER) membrane.

How does AGPAT5’s function relate to lipid droplet dynamics

AGPAT5’s role influences the dynamics and formation of lipid droplets in cells.

Can AGPAT5’s role intersect with cellular growth pathways

Yes, AGPAT5’s activity can influence cellular growth and proliferation through lipid metabolism.

Does AGPAT5’s function vary across different cell types

Yes, AGPAT5’s role can vary based on the specific cellular context and function.

How does AGPAT5 contribute to membrane structure

AGPAT5’s activity contributes to maintaining the structural integrity of cellular membranes.