Recombinant Human AHI1 Protein (Met1-His350), N-His-tagged

• Cat.No.: AHI1-0454H
• Product Overview: Recombinant human AHI1 (Met1-His350) was fused with the N-His tag and was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 42.08 kDa
• Protein Length: Met1-His350
• Purity: >90% as determined by SDS-PAGE
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: 0.01M PBS, pH 7.4, 0.02% NLS
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: AHI1 Abelson helper integration site 1 [ Homo sapiens (human) ]
• Official Symbol: AHI1
• Synonyms: AHI1; Abelson helper integration site 1; Abelson helper integration site; jouberin; FLJ20069; JBTS3; Jouberin; ORF1; contatins SH3 and WD40 domains; abelson helper integration site 1 protein homolog; AHI-1; dJ71N10.1; FLJ14023; DKFZp686J1653
• Gene ID: 54806
• mRNA Refseq: NM_001134830
• Protein Refseq: NP_001128302
• MIM: 608894
• UniProt ID: Q8N157

Reviews

05/25/2022

We are thoroughly impressed with the proficiency of this lab protein, and it has undoubtedly elevated the caliber of our research investigations.

12/12/2021

I'd give this protein ten stars if I could – it's that good.

03/23/2020

This protein has made my experiments feel like a walk in the park – I highly recommend it!

11/02/2019

Kudos to the developers of this recombination protein – it's a brilliant scientific tool.

07/17/2018

The lab protein product has proven to be a reliable and indispensable tool in our laboratory, delivering reproducible results with utmost precision.

Q&As

Can AHI1 gene editing technologies, such as CRISPR/Cas9, be used for potential therapeutic purposes?

Gene editing technologies like CRISPR/Cas9 hold promise for potential therapeutic interventions in AHI1-related disorders. However, further research is needed to address safety and ethical considerations.

What animal models are commonly used to study the function of the AHI1 gene?

Mouse models with AHI1 gene mutations have been widely used to study the function of the AHI1 protein and its role in ciliopathies.

How is the AHI1 protein connected to ciliary transport and signaling defects in ciliopathies?

AHI1 mutations disrupt ciliary transport and signaling, leading to defective cilia formation and function. These defects contribute to the pathogenesis of ciliopathies, such as Joubert syndrome.

How does the AHI1 protein contribute to vesicle trafficking within the cell?

The AHI1 protein is involved in regulating the formation and function of primary cilia, which act as sensory organelles and are essential for vesicle trafficking and cell signaling.

What experimental techniques are commonly used to study the AHI1 protein's interactions?

Techniques such as co-immunoprecipitation, yeast two-hybrid assays, and proximity-based labeling methods are commonly used to study the interactions of the AHI1 protein with other cellular proteins.

How do AHI1 mutations contribute to the pathogenesis of Joubert syndrome?

AHI1 mutations disrupt the normal function of the AHI1 protein, affecting ciliogenesis and intraflagellar transport, which are essential processes in the development of the brain's cerebellum and brainstem. This disruption leads to the characteristic features of Joubert syndrome.

What are the implications of AHI1 mutations in human diseases?

AHI1 mutations have been linked to a spectrum of human disorders, including Joubert syndrome and nephronophthisis-related ciliopathies.

What are the known interacting partners of the AHI1 protein?

The AHI1 protein is known to interact with proteins involved in intraflagellar transport (IFT), such as IFT122 and IFT139, as well as other ciliary-associated proteins.

How is the AHI1 gene regulated at the transcriptional level?

The transcription of the AHI1 gene can be influenced by various factors, including transcription factors and epigenetic modifications.

What is the function of the AHI1 protein in cellular processes?

The AHI1 protein plays a critical role in various cellular processes, such as ciliogenesis, cell signaling, and vesicle trafficking.