Recombinant Human AHNAK 293 Cell Lysate

• Cat.No.: AHNAK-8963HCL

Specification

• Description: Antigen standard for AHNAK nucleoprotein (AHNAK), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AHNAK AHNAK nucleoprotein [ Homo sapiens ]
• Official Symbol: AHNAK
• Synonyms: AHNAK; AHNAK nucleoprotein; AHNAK nucleoprotein (desmoyokin); neuroblast differentiation-associated protein AHNAK; desmoyokin; MGC5395; AHNAK-related; AHNAKRS
• Gene ID: 79026
• mRNA Refseq: NM_001620
• Protein Refseq: NP_001611
• MIM: 103390
• UniProt ID: Q09666
• Chromosome Location: 11q12-q13
• Function: protein binding

Reviews

02/19/2022

The efficacy and dependability of this lab protein underscore its role as a pivotal component in our laboratory's scientific pursuits.

09/09/2021

This protein rocks! It's made our experiments so much easier.

10/25/2020

Prefect results demonstartion. Love it!

02/09/2019

Seriously impressed with the results from this lab protein.

06/04/2018

We confidently endorse this lab protein for its ability to produce dependable and replicable data, aligning with rigorous scientific standards.

Q&As

How does AHNAK contribute to cellular responses to mechanical stress and stretch?

AHNAK has been linked to mechanosensitive signaling pathways, and its interaction with proteins involved in the cytoskeleton and membrane repair suggests a role in cellular responses to mechanical stress and stretch.

What are the known structural domains of the AHNAK protein?

AHNAK contains several functional domains, including the AHNAK repeat domain (ARD), the ankyrin repeat domain (ANK), and the spectrin repeat domain (SRD).

How is AHNAK involved in the regulation of lipid metabolism and lipid droplet dynamics?

AHNAK has been shown to interact with proteins involved in lipid metabolism and lipid droplet formation, suggesting a role in lipid droplet dynamics and lipid homeostasis within cells.

How does AHNAK contribute to the regulation of cytoskeletal dynamics?

AHNAK interacts with actin and microtubule networks, influencing cytoskeletal dynamics and cell motility.

Are there any post-translational modifications of AHNAK other than phosphorylation?

Yes, AHNAK can also undergo acetylation, glycosylation, and ubiquitination, which can modulate its function and stability.

What role does AHNAK play in cancer development and progression?

AHNAK has been implicated in cancer metastasis, invasion, and cell migration, suggesting its potential role as a metastasis-associated protein in certain cancers.

Can AHNAK modulate the activity of ion channels, and if so, how?

Yes, AHNAK interacts with voltage-gated potassium channels (Kv1.5), regulating their localization at the plasma membrane and potentially affecting their activity.

What is the role of AHNAK in membrane repair processes?

AHNAK is recruited to the site of membrane damage, where it interacts with annexins and facilitates membrane resealing, contributing to membrane repair.

How is AHNAK involved in calcium signaling?

AHNAK binds to and modulates the activity of proteins involved in calcium signaling pathways, such as annexins and the plasma membrane calcium ATPase (PMCA).

How does AHNAK contribute to cell adhesion and migration?

AHNAK interacts with various adhesion proteins and the cytoskeleton, playing a role in stabilizing cell-cell and cell-matrix interactions and modulating cell migration.