Recombinant Human AHNAK protein, His & T7-tagged

• Cat.No.: AHNAK-1699H
• Product Overview: Recombinant Human AHNAK aa. (Met1~Asp300 (Accession # Q09666)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a large (700 kDa) structural scaffold protein consisting of a central domain with 128 aa repeats. The encoded protein may play a role in such diverse processes as blood-brain barrier formation, cell structure and migration, cardiac calcium channel regulation, and tumor metastasis. A much shorter variant encoding a 17 kDa isoform exists for this gene, and the shorter isoform initiates a feedback loop that regulates alternative splicing of this gene.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Met1~Asp300 (Accession # Q09666)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 5.3

Gene Information

• Gene Name: AHNAK AHNAK nucleoprotein [ Homo sapiens (human) ]
• Official Symbol: AHNAK
• Synonyms: AHNAK; AHNAK nucleoprotein; PM227; AHNAKRS; neuroblast differentiation-associated protein AHNAK; AHNAK-related; desmoyokin
• Gene ID: 79026
• mRNA Refseq: NM_001346445.1
• Protein Refseq: NP_001333374.1
• UniProt ID: Q09666

Reviews

02/19/2022

The efficacy and dependability of this lab protein underscore its role as a pivotal component in our laboratory's scientific pursuits.

09/09/2021

This protein rocks! It's made our experiments so much easier.

10/25/2020

Prefect results demonstartion. Love it!

02/09/2019

Seriously impressed with the results from this lab protein.

06/04/2018

We confidently endorse this lab protein for its ability to produce dependable and replicable data, aligning with rigorous scientific standards.

Q&As

How does AHNAK contribute to cellular responses to mechanical stress and stretch?

AHNAK has been linked to mechanosensitive signaling pathways, and its interaction with proteins involved in the cytoskeleton and membrane repair suggests a role in cellular responses to mechanical stress and stretch.

What are the known structural domains of the AHNAK protein?

AHNAK contains several functional domains, including the AHNAK repeat domain (ARD), the ankyrin repeat domain (ANK), and the spectrin repeat domain (SRD).

How is AHNAK involved in the regulation of lipid metabolism and lipid droplet dynamics?

AHNAK has been shown to interact with proteins involved in lipid metabolism and lipid droplet formation, suggesting a role in lipid droplet dynamics and lipid homeostasis within cells.

How does AHNAK contribute to the regulation of cytoskeletal dynamics?

AHNAK interacts with actin and microtubule networks, influencing cytoskeletal dynamics and cell motility.

Are there any post-translational modifications of AHNAK other than phosphorylation?

Yes, AHNAK can also undergo acetylation, glycosylation, and ubiquitination, which can modulate its function and stability.

What role does AHNAK play in cancer development and progression?

AHNAK has been implicated in cancer metastasis, invasion, and cell migration, suggesting its potential role as a metastasis-associated protein in certain cancers.

Can AHNAK modulate the activity of ion channels, and if so, how?

Yes, AHNAK interacts with voltage-gated potassium channels (Kv1.5), regulating their localization at the plasma membrane and potentially affecting their activity.

What is the role of AHNAK in membrane repair processes?

AHNAK is recruited to the site of membrane damage, where it interacts with annexins and facilitates membrane resealing, contributing to membrane repair.

How is AHNAK involved in calcium signaling?

AHNAK binds to and modulates the activity of proteins involved in calcium signaling pathways, such as annexins and the plasma membrane calcium ATPase (PMCA).

How does AHNAK contribute to cell adhesion and migration?

AHNAK interacts with various adhesion proteins and the cytoskeleton, playing a role in stabilizing cell-cell and cell-matrix interactions and modulating cell migration.