Recombinant Human AK3 protein, GST-tagged

• Cat.No.: AK3-301589H
• Product Overview: Recombinant Human AK3 (1-227 aa) protein, fused to GST tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: GST
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization.
• Protein length: Met1-Pro227
• AA Sequence: MGASARLLRAVIMGAPGSGKGTVSS RITTHFELKHLSSGDLLRDNMLRGT EIGVLAKAFIDQGKLIPDDVMTRLA LHELKNLTQYSWLLDGFPRTLPQAE ALDRAYQIDTVINLNVPFEVIKQRL TARWIHPASGRVYNIEFNPPKTVGI DDLTGEPLIQREDDKPETVIKRLKA YEDQTKPVLEYYQKKGVLETFSGTE TNKIWPYVYAFLQTKVPQRSQKASV TP
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Stability: Store for up to 12 months at -20°C to -80°C as lyophilized powder.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.

Gene Information

• Gene Name: AK3 adenylate kinase 3 [ Homo sapiens ]
• Official Symbol: AK3
• Synonyms: AK3; adenylate kinase 3; adenylate kinase 3 like 1 , adenylate kinase 6 , AK3L1, AK6; GTP:AMP phosphotransferase, mitochondrial; AKL3L1; adenylate kinase 3 alpha-like 1; adenylate kinase 6, adenylate kinase 3 like 1; AK6; FIX; AK3L1; AKL3L
• Gene ID: 50808
• mRNA Refseq: NM_001199852
• Protein Refseq: NP_001186781
• MIM: 609290
• UniProt ID: Q9UIJ7

Reviews

10/16/2018

The AK3 protein epitomizes unparalleled quality, positioning itself as an impeccable solution that precisely caters to my experimental requisites.

04/02/2018

Its exceptional integrity and consistency instill utmost confidence in obtaining precise and reliable outcomes, thereby propelling my research endeavors to uncharted levels of success.

09/16/2017

Their exemplary technical support team stands poised to address any concerns or hurdles that may arise along my experimental path.

06/17/2016

In addition to its exceptional quality, the manufacturer behind the AK3 protein sets a new standard for excellence with their unwavering dedication to customer satisfaction.

Q&As

Can you provide any insights into the structure of AK3 protein?

AK3 protein is a homodimer, meaning it is composed of two identical subunits. Each subunit consists of about 200 amino acids and contains a nucleotide binding domain that enables AK3 to bind to ATP, ADP, and AMP. The protein structure also contains a highly conserved sequence motif found in other adenylate kinases.

What are some of the diseases or conditions associated with dysregulation of AK3 protein?

Dysregulation of AK3 protein has been implicated in various diseases and conditions. Some examples include cancer, where altered AK3 expression has been observed in certain types of tumors, suggesting its involvement in cancer progression.

Does AK3 protein have any interaction partners in the cell?

AK3 protein has been found to interact with other molecules within the cell. For example, it can interact with adenine nucleotides like ATP, ADP, and AMP, as well as other enzymes involved in energy metabolism. Additionally, studies have suggested potential protein-protein interactions between AK3 and other mitochondrial proteins involved in various cellular processes.

Where is AK3 protein found in the body?

AK3 protein is primarily found in the mitochondria, which are the energy-producing organelles within cells. It is more specifically located in the mitochondrial intermembrane space.

Are there any other notable features or functions of AK3 protein?

One notable feature of AK3 protein is its ability to reversibly form disulfide bonds under oxidative conditions, which may regulate its activity and localization within the mitochondria. AK3 protein has also been implicated in protecting cells against oxidative stress and apoptosis, suggesting additional roles beyond its energy metabolism function.

Can AK3 protein be used as a diagnostic or prognostic marker for any diseases?

There is ongoing research investigating the potential use of AK3 protein as a diagnostic or prognostic marker for certain diseases. For instance, altered expression levels of AK3 have been found in various cancers, indicating its potential as a biomarker for tumor detection or progression. However, further studies are needed to validate its clinical utility as a diagnostic or prognostic marker.

Are there any drugs or therapeutic strategies targeting AK3 protein?

Currently, there are no specific drugs or therapeutic strategies targeting AK3 protein directly. However, understanding the role of AK3 in disease processes may help identify potential targets for drug development and therapeutic interventions in the future.

Are there any ongoing research studies or future directions in AK3 protein research?

AK3 protein continues to be an active area of research. Future studies may focus on elucidating the precise molecular mechanisms underlying its role in cancer, mitochondrial disorders, and neurodegenerative diseases. Additionally, researchers may investigate potential therapeutic strategies targeting AK3 or its associated pathways as a means to treat related disorders.

Are there any known genetic variations or mutations in the AK3 gene?

Yes, there have been identified genetic variations and mutations in the AK3 gene. Some variations may alter the activity or expression levels of AK3 protein, potentially affecting its function. These genetic variations can have implications for various diseases and conditions.

Is AK3 protein involved in any specific signaling pathways?

While AK3 protein primarily functions in cellular energy metabolism, it can also indirectly influence signaling pathways through the regulation of AMP levels. AMP can activate the AMP-activated protein kinase (AMPK) pathway, which plays a crucial role in cellular energy sensing and regulation. Thus, by modulating AMP levels, AK3 protein may indirectly affect signaling pathways involved in metabolism, cell proliferation, and stress response.