Recombinant Human AKAP8 cell lysate

• Cat.No.: AKAP8-46HCL

Specification

• Description: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.
• Species: Human
• Size: 100 ul
• Storage Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
• Applications: Western Blot

Gene Information

• Gene Name: AKAP8 A kinase (PRKA) anchor protein 8 [ Homo sapiens ]
• Official Symbol: AKAP8
• Synonyms: AKAP8; A kinase (PRKA) anchor protein 8; A-kinase anchor protein 8; A kinase anchor protein; 95kDa; AKAP95; DKFZp586B1222; A-kinase anchor protein, 95kDa; AKAP-8; AKAP 95; AKAP-95
• Gene ID: 10270
• mRNA Refseq: NM_005858
• Protein Refseq: NP_005849
• MIM: 604692
• UniProt ID: O43823
• Chromosome Location: 19p13.1-q12
• Pathway: G Protein Signaling Pathways, organism-specific biosystem; TNF-alpha/NF-kB Signaling Pathway, organism-specific biosystem
• Function: DNA binding; double-stranded DNA binding; metal ion binding; protein kinase A regulatory subunit binding; zinc ion binding

Reviews

01/09/2022

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12/17/2019

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10/24/2019

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08/13/2017

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Q&As

Are there any known drugs or compounds that specifically target AKAP8?

At present, there are no known drugs or compounds specifically targeting AKAP8. However, as our knowledge of AKAP8's roles and interactions expands, it is possible that specific inhibitors or modulators could be developed in the future.

Where is AKAP8 localized within the cell?

AKAP8 is primarily localized within the nucleus of the cell. It is found in both the nucleoplasm and at specific sites within the nucleus, such as the nucleolus and nuclear matrix. AKAP8 interacts with various nuclear proteins and structures to regulate their activities and facilitate cellular functions.

Does AKAP8 have any role in DNA replication and repair?

Yes, AKAP8 is involved in DNA replication and repair processes. It interacts with proteins involved in DNA replication initiation and progression, such as DNA polymerase α and the minichromosome maintenance complex. AKAP8 also participates in DNA repair pathways by interacting with DNA repair factors, such as the nucleotide excision repair protein XPA.

Are there any genetic disorders associated with mutations or dysregulation of AKAP8?

At present, there are no known human genetic disorders specifically attributed to mutations in AKAP8. However, dysregulation of AKAP8 expression or function has been associated with various diseases, including cancer, neurodevelopmental disorders, and cardiovascular conditions. Further research is needed to determine the extent of AKAP8's involvement in these disorders and potential therapeutic applications.

What are potential future directions for AKAP8 research?

Future research on AKAP8 may involve further elucidation of its intricate molecular mechanisms and protein interactions. Investigating its role in specific diseases and exploring therapeutic strategies targeting AKAP8 could be potential areas of focus. Additionally, understanding how AKAP8 contributes to development, differentiation, and tissue homeostasis may provide insights into its broader physiological functions.

Can AKAP8 be used as a diagnostic marker for certain diseases?

While AKAP8 dysregulation has been observed in certain diseases, it is yet to be established as a reliable diagnostic marker for specific conditions. However, the identification of AKAP8 expression patterns or protein levels in certain tissues or patient samples may provide valuable insights and potential diagnostic indicators in the future.

Are there any animal models available to study AKAP8?

Yes, animal models with altered expression or function of AKAP8 have been developed to study its roles in various biological processes. These models include mice with AKAP8 gene deletions or mutations. By studying these models, researchers aim to unravel the specific functions of AKAP8 and its contributions to disease development and progression.

Does AKAP8 have any involvement in DNA packaging and chromatin structure?

Yes, AKAP8 contributes to DNA packaging and chromatin structure through its interactions with chromatin remodeling enzymes. It facilitates the recruitment of these enzymes to specific gene promoters, resulting in alterations to chromatin structure and accessibility for transcription factors and regulatory proteins.

Can AKAP8 affect cellular signaling pathways other than PKA signaling?

Yes, AKAP8 has been shown to interact with other signaling molecules and modulate their activities. For instance, AKAP8 can associate with protein phosphatase 2A (PP2A) and regulate its localization and function in the nucleus. AKAP8 has also been reported to influence the activity of the c-Jun N-terminal kinase (JNK) signaling pathway, which is involved in cellular stress responses and apoptosis.

Are there any genetic variations or polymorphisms in the AKAP8 gene?

There is limited information available on genetic variations in the AKAP8 gene. However, some studies have reported single nucleotide polymorphisms (SNPs) in AKAP8 that may be associated with certain diseases or traits. For example, a SNP in AKAP8 has been associated with an increased risk of developing ischemic stroke. Further research is needed to explore the potential significance of these variations and their impact on AKAP8 function and disease susceptibility.

How does AKAP8 contribute to cell cycle progression?

AKAP8 is involved in various stages of the cell cycle and helps coordinate cell cycle progression. It interacts with cyclins and cyclin-dependent kinases (CDKs) to regulate their activities and facilitate cell cycle transitions. AKAP8 also plays a role in the regulation of mitotic spindle assembly and chromosome segregation during cell division.

Are there any specific diseases or disorders associated with AKAP8 dysfunction?

Research suggests that abnormalities in AKAP8 function may be associated with certain diseases. For example, altered expression of AKAP8 has been observed in various cancers, including breast, ovarian, and colorectal cancers. Dysfunction of AKAP8 in these cases may contribute to abnormal cell proliferation, tumor growth, and metastasis.

Can AKAP8 be targeted therapeutically for certain diseases?

Targeting AKAP8 may hold potential for therapeutic interventions in diseases where its dysregulation plays a significant role. For example, in certain cancers, inhibiting AKAP8's interactions with other proteins or disrupting its localization could potentially hinder tumor growth and metastasis. However, further research is needed to fully understand the complex functions of AKAP8 and its potential as a therapeutic target.

What are some current areas of research on AKAP8?

Current research on AKAP8 spans various fields, including cancer biology, neurodevelopmental disorders, gene regulation, and cellular signaling. Some areas of active investigation include understanding the molecular mechanisms by which AKAP8 influences gene expression, characterizing its interactions with other proteins in different cellular contexts, and exploring its potential as a therapeutic target in diseases such as cancer and neurological disorders.