Recombinant Human ALKBH1 293 Cell Lysate

• Cat.No.: ALKBH1-8903HCL

Specification

• Description: Antigen standard for alkB, alkylation repair homolog 1 (E. coli) (ALKBH1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ALKBH1 alkB, alkylation repair homolog 1 (E. coli) [ Homo sapiens ]
• Official Symbol: ALKBH1
• Synonyms: ALKBH1; alkB, alkylation repair homolog 1 (E. coli); alkB, alkylation repair homolog (E. coli) , ALKBH; alkylated DNA repair protein alkB homolog 1; ABH; alkB; hABH; DNA lyase ABH1; alkylation repair, alkB homolog; alpha-ketoglutarate-dependent dioxygenase ABH1; ABH1; ALKBH
• Gene ID: 8846
• mRNA Refseq: NM_006020
• Protein Refseq: NP_006011
• MIM: 605345
• UniProt ID: Q13686
• Chromosome Location: 14q24
• Function: DNA-(apurinic or apyrimidinic site) lyase activity; chemoattractant activity; ferrous iron binding; lyase activity; metal ion binding; methylcytosine dioxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen

Reviews

12/17/2020

Can meet the specific experimental requirements and research purposes when used in experiments.

05/16/2020

The products are available in a wide range of specifications for different sizes and experimental designs.

04/18/2020

When ALKBH1 was used in the experiment, clear and consistent results could be obtained, which was convenient for data analysis and interpretation.

12/24/2019

Long half-life and is able to maintain stability and reliability during the experiment.

Q&As

How does ALKBH1 participate in DNA base repair and oxidative stress?

ALKBH1 can remove oxidative components and methylation markers from DNA, and is involved in DNA base repair and oxidative stress.

How to evaluate the expression and role of ALKBH1 in liver cancer?

By studying the expression and function of ALKBH1 in liver cancer tissue, we can understand its role in the treatment of liver cancer.

In which tissues is ALKBH1 expressed?

ALKBH1 is mainly expressed in liver, spleen, myocardium and other tissues.

What is the role of ALKBH1 in the occurrence and development of gastric cancer?

ALKBH1 plays a key role in the development and progression of gastric cancer, which may be related to the regulation of DNA repair and apoptosis.

What is the significance of changes in ALKBH1 expression for the prediction of metabolic diseases?

The study on the expression level of ALKBH1 in metabolic diseases can provide new ideas for the prediction and treatment of metabolic diseases.

How does ALKBH1 affect the immune system and inflammatory response?

It can influence the function of the immune system and the occurrence of inflammatory responses, regulating cell state by removing oxidative components and methylation markers.

What is the role of ALKBH1 in nervous system and related diseases?

It has been shown to play an important role in neurological and related disorders, including mild cognitive impairment.

Is ALKBH1 expression regulated by environmental factors and endogenous signals?

The expression may be regulated by various environmental factors and endogenous signals, including chemical stimulation and oxidative stress.

What is the role of ALKBH1 in DNA damage repair?

ALKBH1 plays a key role in DNA damage and repair, repairing 1- or 3-methyladenine in DNA.

What is the role of ALKBH1 in cardiovascular disease?

This protein plays an important role in the occurrence and development of cardiovascular diseases, which may be related to lipid metabolism and pathway regulation.

Which method does ALKBH1 in mammalian stem cell reprogramming?

ALKBH1 plays an important role in mammalian stem cell reprogramming, promoting stem cell proliferation and differentiation.

Which effect of ALKBH1 mutation on metabolic capacity?

The mutation may affect metabolic capacity and cause liver disease, diabetes, obesity and other related diseases.

How does ALKBH1 participate in DNA methylation repair?

It can remove 1- or 3-methyladenine from DNA and is involved in DNA methylation repair and DNA repair processes.

What diseases are ALKBH1 mutations associated with?

The mutation may be associated with cancer, cardiovascular disease, liver disease, etc.

What is the enzyme activity mechanism of ALKBH1?

ALKBH1 repairs DNA damage by removing 1- or 3-methyladenine from DNA double-stranded DNA in an oxygen-dependent manner.

How to study the expression and role of ALKBH1 in certain diseases?

The expression and role of ALKBH1 in certain diseases can be comprehensively evaluated through comparative omics, immunopathology, molecular biology and other research methods, so as to provide references for disease prediction and treatment.

Which cell signal transduction pathways does ALKBH1 participate in?

ALKBH1 is involved in a variety of cell signaling pathways, including NF-κB.