Recombinant Mouse Alkbh1 Protein, Myc/DDK-tagged

• Cat.No.: Alkbh1-1599M
• Product Overview: Purified recombinant protein of mouse full-length alkB homolog 1, histone H2A dioxygenase (Alkbh1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Dioxygenase that acts as on nucleic acids, such as DNA and tRNA. Requires molecular oxygen, alpha-ketoglutarate and iron. A number of activities have been described for this dioxygenase, but recent results suggest that it mainly acts as on tRNAs and mediates their demethylation or oxidation depending on the context and subcellular compartment. Mainly acts as a tRNA demethylase by removing N1-methyladenine from various tRNAs, with a preference for N1-methyladenine at position 58 (m1A58) present on a stem loop structure of tRNAs. Acts as a regulator of translation initiation and elongation in response to glucose deprivation: regulates both translation initiation, by mediating demethylation of tRNA(Met), and translation elongation, N1-methyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation. In mitochondrion, specifically interacts with mt-tRNA(Met) and mediates oxidation of mt-tRNA(Met) methylated at cytosine(34) to form 5-formylcytosine (f5c) at this position. mt-tRNA(Met) containing the f5c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation. Specifically demethylates DNA methylated on the 6th position of adenine (N6-methyladenosine) DNA. N6-methyladenosine (m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing. Demethylates mRNAs containing N3-methylcytidine modification. Also able to repair alkylated single-stranded DNA by oxidative demethylation, but with low activity. Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketboglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product. DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 43.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Alkbh1 alkB homolog 1, histone H2A dioxygenase [ Mus musculus (house mouse) ]
• Official Symbol: Alkbh1
• Synonyms: ALKBH1; alkB, alkylation repair homolog 1 (E. coli); alkylated DNA repair protein alkB homolog 1; DNA lyase ABH1; alpha-ketoglutarate-dependent dioxygenase ABH1; Abh; alkB; hABH; Alkbh; 2700073G19Rik
• Gene ID: 211064
• mRNA Refseq: NM_001102565
• Protein Refseq: NP_001096035
• UniProt ID: P0CB42

Reviews

12/17/2020

Can meet the specific experimental requirements and research purposes when used in experiments.

05/16/2020

The products are available in a wide range of specifications for different sizes and experimental designs.

04/18/2020

When ALKBH1 was used in the experiment, clear and consistent results could be obtained, which was convenient for data analysis and interpretation.

12/24/2019

Long half-life and is able to maintain stability and reliability during the experiment.

Q&As

How does ALKBH1 participate in DNA base repair and oxidative stress?

ALKBH1 can remove oxidative components and methylation markers from DNA, and is involved in DNA base repair and oxidative stress.

How to evaluate the expression and role of ALKBH1 in liver cancer?

By studying the expression and function of ALKBH1 in liver cancer tissue, we can understand its role in the treatment of liver cancer.

In which tissues is ALKBH1 expressed?

ALKBH1 is mainly expressed in liver, spleen, myocardium and other tissues.

What is the role of ALKBH1 in the occurrence and development of gastric cancer?

ALKBH1 plays a key role in the development and progression of gastric cancer, which may be related to the regulation of DNA repair and apoptosis.

What is the significance of changes in ALKBH1 expression for the prediction of metabolic diseases?

The study on the expression level of ALKBH1 in metabolic diseases can provide new ideas for the prediction and treatment of metabolic diseases.

How does ALKBH1 affect the immune system and inflammatory response?

It can influence the function of the immune system and the occurrence of inflammatory responses, regulating cell state by removing oxidative components and methylation markers.

What is the role of ALKBH1 in nervous system and related diseases?

It has been shown to play an important role in neurological and related disorders, including mild cognitive impairment.

Is ALKBH1 expression regulated by environmental factors and endogenous signals?

The expression may be regulated by various environmental factors and endogenous signals, including chemical stimulation and oxidative stress.

What is the role of ALKBH1 in DNA damage repair?

ALKBH1 plays a key role in DNA damage and repair, repairing 1- or 3-methyladenine in DNA.

What is the role of ALKBH1 in cardiovascular disease?

This protein plays an important role in the occurrence and development of cardiovascular diseases, which may be related to lipid metabolism and pathway regulation.

Which method does ALKBH1 in mammalian stem cell reprogramming?

ALKBH1 plays an important role in mammalian stem cell reprogramming, promoting stem cell proliferation and differentiation.

Which effect of ALKBH1 mutation on metabolic capacity?

The mutation may affect metabolic capacity and cause liver disease, diabetes, obesity and other related diseases.

How does ALKBH1 participate in DNA methylation repair?

It can remove 1- or 3-methyladenine from DNA and is involved in DNA methylation repair and DNA repair processes.

What diseases are ALKBH1 mutations associated with?

The mutation may be associated with cancer, cardiovascular disease, liver disease, etc.

What is the enzyme activity mechanism of ALKBH1?

ALKBH1 repairs DNA damage by removing 1- or 3-methyladenine from DNA double-stranded DNA in an oxygen-dependent manner.

How to study the expression and role of ALKBH1 in certain diseases?

The expression and role of ALKBH1 in certain diseases can be comprehensively evaluated through comparative omics, immunopathology, molecular biology and other research methods, so as to provide references for disease prediction and treatment.

Which cell signal transduction pathways does ALKBH1 participate in?

ALKBH1 is involved in a variety of cell signaling pathways, including NF-κB.