Recombinant Human ALKBH3, GST-tagged

• Cat.No.: ALKBH3-9584H
• Product Overview: Recombinant Human ALKBH3 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Description: The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).
• Source: E.coli
• Species: Human
• Tag: GST
• Protein length: C-term-139a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: ALKBH3 alkB, alkylation repair homolog 3 (E. coli) [ Homo sapiens ]
• Official Symbol: ALKBH3
• Synonyms: ALKBH3; alkB, alkylation repair homolog 3 (E. coli); alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; DEPC 1; prostate cancer antigen 1; prostate cancer antigen-1; alkylated DNA repair protein alkB homolog 3; ABH3; PCA1; DEPC1; DEPC-1; FLJ43614; MGC118790; MGC118792; MGC118793
• Gene ID: 221120
• mRNA Refseq: NM_139178
• Protein Refseq: NP_631917
• MIM: 610603
• UniProt ID: Q96Q83
• Chromosome Location: 11p11.2
• Pathway: ABH3 mediated Reversal of Alkylation Damage, organism-specific biosystem; DNA Damage Reversal, organism-specific biosystem; DNA Repair, organism-specific biosystem; Reversal of Alkylation Damage By DNA Dioxygenases, organism-specific biosystem
• Function: DNA-N1-methyladenine dioxygenase activity; L-ascorbic acid binding; cytosine C-5 DNA demethylase activity; ferrous iron binding; metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular o

Reviews

04/30/2021

Structure analysis by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy showed that ALKBH3 was stable.

04/28/2021

Prone to induce immune responses.

05/11/2019

Stable under special conditions.

Q&As

What diseases is the mutation of ALKBH3 gene associated with?

ALKBH3 gene mutation is associated with colon and rectal cancer.

What factors are related to the stability of ALKBH3 recombinant protein?

The stability of ALKBH3 recombinant protein is related to freezing temperature, pH value and ionic strength.

What are the biological functions of human ALKBH3 recombinant protein?

ALKBH3 is a DNA repair enzyme that repairs methyl groups along DNA strands.

Is ALKBH3 mutation associated with recurrent liver cancer?

No studies have shown that ALKBH3 mutations are associated with recurrent liver cancer.

Is ALKBH3 gene involved in drug metabolism?

At present, there is no clear study that ALKBH3 gene is related to drug metabolism.

Can ALKBH3 gene be used as a molecular marker for the prognosis of liver cancer?

ALKBH3 gene can be used as one of the molecular markers of liver cancer prognosis.

Does ALKBH3 have immunomodulatory effects?

There is no clear evidence that ALKBH3 recombinant protein has immunomodulatory effects.

Are polymorphisms of ALKBH3 gene associated with any diseases?

ALKBH3 gene polymorphism is associated with various cancers such as lung cancer, esophageal cancer and colon cancer.

Does ALKBH3 mutation affect DNA damage repair function?

Mutations in ALKBH3 may affect the function of DNA damage repair.

What is the stability of ALKBH3 recombinant protein?

ALKBH3 recombinant protein should be stored in a -80℃ refrigerator to ensure stability.

What is the role of ALKBH3 recombinant protein in cancer treatment?

ALKBH3 recombinant protein can help predict the therapeutic effect and prognosis of some cancers.

Does ALKBH3 gene affect human metabolism?

ALKBH3 has not been found to affect human metabolic capacity.

Does ALKBH3 have an effect on the immune system?

No studies have shown that ALKBH3 has an important effect on the immune system.

Does the recommended dosage of ALKBH3 recombinant protein?

There are no clear dosage guidelines at present, and further research is needed to determine.

Is ALKBH3 related to treatment response of liver cancer?

ALKBH3 mutation may affect the treatment response of liver cancer.

Can ALKBH3 gene variation be used as an early warning indicator of cancer?

There is no strong evidence that the ALKBH3 gene variant can be used as an early warning indicator of cancer.

Can ALKBH3 recombinant protein be used as a target for cancer treatment?

The recombinant ALKBH3 protein may be a potential target for the treatment of some cancers, but further research is needed to confirm it.