Recombinant Human ALKBH5 Protein, Flag-tagged

• Cat.No.: ALKBH5-32H
• Product Overview: Recombinant ALKBH5 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_060228.3) with an N-terminal FLAG-Tag. The molecular weight of ALKBH5 is 45.5 kDa.

Specification

• Description: N6-methylated adenine (m6A) is prevalent in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also appears in other RNA molecules, such as primary miRNA (pri-miRNAs).
  ALKBH5 (AlkB Homolog 5, RNA Demethylase, also known as OFOXD1, ABH5) specifically demethylates m6A RNA. ALKBH5 significantly affects mRNA export and RNA metabolism as well as the assembly of mRNA processing factors in nuclear speckles via demethylation activity.
• Source: Insect Cells
• Species: Human
• Tag: Flag
• Form: Recombinant ALKBH5 protein is supplied at a concentration of 0.3 µg/µl in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP.
• Molecular Mass: 45.5 kDa
• Purity: > 85%
• Applications: Recombinant ALKBH5 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
  3 µM m6A ssDNA oligos (DNA sequence: 5’-GTTGCCTGTTCGTGTTGG/m6A/ CTTGCCTGT-3’) were incubated with ALKBH5 protein in reaction buffer including 50 mM HEPES pH 7.5, 100 µM 2OG, 100 µM Ascorbate, 50 µM (NH4)2Fe(SO4)2·6H2O and 1 mM TCEP for 2 hours at room temperature. Dot-Blot was used for activity detection.
• Notes: This product is for research use only and is not for use in diagnostic procedures.
• Stability: This product is stable for 6 months from data of arrival.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Gene Information

• Gene Name: ALKBH5 alkB, alkylation repair homolog 5 (E. coli) [ Homo sapiens ]
• Official Symbol: ALKBH5
• Synonyms: ALKBH5; FLJ20308; ABH5; OFOXD; OFOXD1
• Gene ID: 54890
• mRNA Refseq: NM_017758
• Protein Refseq: NP_060228
• MIM: 613303
• UniProt ID: Q6P6C2

Reviews

06/07/2022

Good stability and durability under the influence of temperature, humidity and light.

01/25/2022

ALKBH5 has good consistency and reliability.

11/16/2019

Immunogenic and allergenic, which is more important for proteins used in drug development.

05/11/2019

This protein does not elicit a response from the immune system.

Q&As

What is the effect of ALKBH5 protein knockout on cell survival and metabolism?

The knockout of ALKBH5 protein can significantly affect multiple biological processes such as cell survival, proliferation and apoptosis, indicating that ALKBH5 protein is necessary for normal physiological regulation of cells.

In what ways can ALKBH5 protein regulate gene expression and transcriptional modification?

The protein can regulate gene expression and transcriptional modification through hybridization mutation and secondary structure change of m6A RNA modification.

How does the m6A demethylation mechanism of ALKBH5 protein work?

This protein can recognize and bind to m6A RNA methylation modification, and then mediate m6A demethylation modification through active sites, thereby regulating metabolic transcription of target RNA.

What biological processes does ALKBH5 play an important role in?

ALKBH5 plays an important role in RNA modification, metabolism and translation.

Does ALKBH5 participate in m6A modification?

Yes, ALKBH5 is a protein involved in m6A modification.

What is the relationship between ALKBH5 protein and metabolic diseases?

ALKBH5 protein is associated with metabolic diseases such as liver cancer and kidney disease, and its function of regulating metabolic balance is an important link in the occurrence and development of these diseases.

Does ALKBH5 protein have splicing isomers? What is the impact?

ALKBH5 protein has a variety of splicing isomers, which have different functions and roles in interaction with other proteins and RNA modification.

What is the effect of ALKBH5 protein on tumorigenesis and development?

ALKBH5 protein plays an important role in the metabolic process of tumors, so its abnormal expression and dysfunction can cause the occurrence and development of tumors.

In which tissues and cells is ALKBH5 protein highly expressed?

ALKBH5 protein is highly expressed in reproductive organs, brain tissue, heart and other tissues and cells.

Is the expression of ALKBH5 protein affected by endogenous factors? What's the connection?

The expression of ALKBH5 protein is affected by a variety of endogenous factors, such as hormone levels and metabolite concentrations, which are related to the function and regulation of ALKBH5 protein.

In which tissues is ALKBH5 expressed most frequently?

ALKBH5 is frequently expressed in human ovary, testis and thyroid tissue.

Is ALKBH5 involved in human germ cell development?

Yes, ALKBH5 is involved in human germ cell development and fertilization.

What are the interactions between ALKBH5 protein and other proteins? What's the biological significance?

ALKBH5 interacts with proteins related to RNA modification, such as m6A methyltransferase and m6A reader protein, which have important biological significance for the stability and precise regulation of RNA modification.

Is there a specific drug targeted treatment plan for ALKBH5?

At present, no specific drug targeting regimen for ALKBH5 has been identified.

Is there an ALKBH5 enhancer or inhibitor?

No enhancers or inhibitors of ALKBH5 have been developed.

Does ALKBH5 need to bind to other proteins to perform its function?

Yes, ALKBH5 usually needs to bind to other RNA-modified proteins to perform its functions.

Is the mutation of ALKBH5 gene associated with any diseases?

No direct correlation has been found between mutations in ALKBH5 and specific diseases.

Does ALKBH5 participate in the genesis and progression of tumors?

Some studies have suggested that ALKBH5 may have some effect on the development and progression of tumors.

What is the structure and function of ALKBH5 protein?

The ALKBH5 protein is a protein that regulates RNA modification and mediates demethylation of m6A RNA methylation.